Current methods to analyze lysosome morphology, positioning, motility and function.
Current methods to analyze lysosome morphology, positioning, motility and function.
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DOI：
10.1111/tra.12839
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发表时间：
2022-05
期刊：
影响因子：
4.5
通讯作者：
Seabra, Miguel C.
中科院分区：
文献类型：
--
作者：
Barral, Duarte C.;Staiano, Leopoldo;Guimas Almeida, Claudia;Cutler, Dan F.;Eden, Emily R.;Futter, Clare E.;Galione, Antony;Marques, Andre R. A.;Medina, Diego Luis;Napolitano, Gennaro;Settembre, Carmine;Vieira, Otilia V.;Aerts, Johannes M. F. G.;Atakpa-Adaji, Peace;Bruno, Gemma;Capuozzo, Antonella;De Leonibus, Elvira;Di Malta, Chiara;Escrevente, Cristina;Esposito, Alessandra;Grumati, Paolo;Hall, Michael J.;Teodoro, Rita O.;Lopes, Susana S.;Luzio, J. Paul;Monfregola, Jlenia;Montefusco, Sandro;Platt, Frances M.;Polishchuck, Roman;De Risi, Maria;Sambri, Irene;Soldati, Chiara;Seabra, Miguel C.
关键词：
自从70多年前发现溶酶体以来，人们对这些细胞器的功能有了很多了解。溶酶体被认为是唯一的降解细胞器，但最近的研究表明，它们在其他几种细胞功能中发挥重要作用，如营养传感，细胞内信号传导和代谢。方法学的进步在产生我们目前关于这个多方面细胞器的生物学知识方面发挥了关键作用。在这篇综述中，我们涵盖了目前用于分析溶酶体形态，定位，运动和功能的方法。我们强调这些方法背后的原则，方法策略及其优势和局限性。为了提取准确的信息并避免误解，我们讨论了识别溶酶体并评估其特征和功能的最佳策略。通过这篇综述，我们的目标是刺激溶酶体研究的数量和质量的增加，以及对细胞器的进一步突破性发现，继续让细胞生物学家感到惊讶和兴奋。溶酶体在其众所周知的降解功能之外发挥着重要作用，包括营养传感，细胞内信号传导和代谢。为了更好地理解这些功能，必须采用最佳方法并了解其优点和局限性。在这里，我们回顾目前用于分析溶酶体形态，定位，运动和功能的方法。这些方法的正确使用将允许对这个迷人的细胞器进行新的发现。
Since the discovery of lysosomes more than 70 years ago, much has been learned about the functions of these organelles. Lysosomes were regarded as exclusively degradative organelles, but more recent research has shown that they play essential roles in several other cellular functions, such as nutrient sensing, intracellular signalling and metabolism. Methodological advances played a key part in generating our current knowledge about the biology of this multifaceted organelle. In this review, we cover current methods used to analyze lysosome morphology, positioning, motility and function. We highlight the principles behind these methods, the methodological strategies and their advantages and limitations. To extract accurate information and avoid misinterpretations, we discuss the best strategies to identify lysosomes and assess their characteristics and functions. With this review, we aim to stimulate an increase in the quantity and quality of research on lysosomes and further ground‐breaking discoveries on an organelle that continues to surprise and excite cell biologists. Lysosomes play essential roles beyond their well‐known degradative function, including nutrient sensing, intracellular signalling and metabolism. To better understand these functions, it is essential to employ the best methodological approaches and know their advantages and limitations. Here, we review current methods used to analyze lysosome morphology, positioning, motility and function. The proper use of these methods will allow new discoveries to be made about this fascinating organelle.
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DOI：
10.1083/jcb.201609033
发表时间：
2017-05-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Allison R;Edgar JR;Pearson G;Rizo T;Newton T;Günther S;Berner F;Hague J;Connell JW;Winkler J;Lippincott-Schwartz J;Beetz C;Winner B;Reid E
通讯作者：
Reid E
影响因子：
5.5
作者：
Bouhamdani N;Comeau D;Turcotte S
通讯作者：
Turcotte S
影响因子：
13.3
作者：
Aits, Sonja;Kricker, Jennifer;Jaattela, Marja
通讯作者：
Jaattela, Marja
影响因子：
4.5
作者：
Bissig, Christin;Hurbain, Ilse;van Niel, Guillaume
通讯作者：
van Niel, Guillaume
影响因子：
5.3
作者：
Brahimi-Horn, M. Christiane;Lacas-Gervais, Sandra;Mazure, Nathalie M.
通讯作者：
Mazure, Nathalie M.