Preferential localization of tyrosine-phosphorylated paxillin in focal adhesions.
Preferential localization of tyrosine-phosphorylated paxillin in focal adhesions.
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酪氨酸磷酸化桩蛋白在粘着斑中的优先定位。
DOI：
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
I. Curtis
中科院分区：
文献类型：
--
作者：
A. Cattelino;S. Cairo;B. Malanchini;I. Curtis
Focal adhesions are sites for integrin-mediated attachment of cultured cells to the extracellular matrix. Localization studies have shown that focal adhesions can be stained by antiphosphotyrosine antibodies, but the role of tyrosine-phosphorylated proteins in focal adhesions is not known. By using ventral plasma membranes prepared from chicken embryo fibroblasts spread on the substrate, we present evidence for the preferential localization of a minor pool of tyrosine-phosphorylated paxillin in focal adhesions. Ventral plasma membranes showed an enrichment in beta 1-integrins, and in several tyrosine-phosphorylated polypeptides, while focal adhesion proteins like vinculin and paxillin, although localized to focal adhesions in ventral plasma membranes, were not particularly enriched in these preparations compared to whole cell lysates. Biochemical and morphological analysis of ventral plasma membranes showed a dramatic increase in the level of tyrosine-phosphorylation of the pool of paxillin localized to the adhesive sites, when compared to the paxillin present in whole cell lysates. The observed preferential localization of tyrosine-phosphorylated paxillin to focal adhesions may represent a general mechanism to compartmentalize focal adhesion components from large non-phosphorylated, cytosolic pools.
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检测正常和 v-Src 转化的 Balb/c 3T3 细胞中的 Src 同源 3 结合蛋白，包括桩蛋白。
DOI：
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发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Weng,Z;Taylor,JA;Turner,CE;Brugge,JS;Seidel-Dugan,C
通讯作者：
Seidel-Dugan,C
DOI：
10.1073/pnas.82.19.6576
发表时间：
1985-01-01
影响因子：
11.1
作者：
MAHER, PA;PASQUALE, EB;SINGER, SJ
通讯作者：
SINGER, SJ
DOI：
10.1073/pnas.91.9.3984
发表时间：
1994-04-26
影响因子：
11.1
作者：
SABE, H;HATA, A;HANAFUSA, H
通讯作者：
HANAFUSA, H
铃蟾肽、加压素和内皮素可快速刺激 Swiss 3T3 细胞中粘着斑相关蛋白桩蛋白的酪氨酸磷酸化。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Zachary,I;Sinnett-Smith,J;Turner,CE;Rozengurt,E
通讯作者：
Rozengurt,E
DOI：
10.1073/pnas.88.19.8392
发表时间：
1991-10-01
影响因子：
11.1
作者：
KORNBERG, LJ;EARP, HS;JULIANO, RL
通讯作者：
JULIANO, RL