Comparative genomics of Vibrio cholerae from Haiti, Asia, and Africa.
Comparative genomics of Vibrio cholerae from Haiti, Asia, and Africa.
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DOI：
10.3201/eid1711.110794
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发表时间：
2011-11
影响因子：
11.8
通讯作者：
V. cholerae Outbreak Genomics Task Force
中科院分区：
文献类型：
--
作者：
Reimer AR;Van Domselaar G;Stroika S;Walker M;Kent H;Tarr C;Talkington D;Rowe L;Olsen-Rasmussen M;Frace M;Sammons S;Dahourou GA;Boncy J;Smith AM;Mabon P;Petkau A;Graham M;Gilmour MW;Gerner-Smidt P;V. cholerae Outbreak Genomics Task Force
来自海地的菌株与来自亚洲和非洲的菌株有着共同的遗传祖先。在2010年秋天海地爆发霍乱之前，伊斯帕尼奥拉岛至少有世纪没有霍乱。对海地疫情和最近返回美国的全球旅行者的临床分离株进行的脉冲场凝胶电泳（PFGE）分析显示，PFGE指纹无法区分。为了更好地探索海地爆发菌株的遗传祖先，我们获得了23个全基因组霍乱弧菌序列：9个分离株来自海地或多米尼加共和国，12个PFGE模式匹配的分离株与亚洲或非洲有关，2个不匹配的离群值来自西半球。全基因组序列和核心基因组单核苷酸多态性的系统发育表明，海地爆发菌株与源自印度和喀麦隆的菌株在遗传上相关。然而，由于在测序的当代分离株中没有发现相同的遗传匹配，海地疫情的确切遗传起源仍然是推测性的。
A strain from Haiti shares genetic ancestry with those from Asia and Africa. Cholera was absent from the island of Hispaniola at least a century before an outbreak that began in Haiti in the fall of 2010. Pulsed-field gel electrophoresis (PFGE) analysis of clinical isolates from the Haiti outbreak and recent global travelers returning to the United States showed indistinguishable PFGE fingerprints. To better explore the genetic ancestry of the Haiti outbreak strain, we acquired 23 whole-genome Vibrio cholerae sequences: 9 isolates obtained in Haiti or the Dominican Republic, 12 PFGE pattern-matched isolates linked to Asia or Africa, and 2 nonmatched outliers from the Western Hemisphere. Phylogenies for whole-genome sequences and core genome single-nucleotide polymorphisms showed that the Haiti outbreak strain is genetically related to strains originating in India and Cameroon. However, because no identical genetic match was found among sequenced contemporary isolates, a definitive genetic origin for the outbreak in Haiti remains speculative.
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通讯作者：
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通讯作者：
Fraser CM