Changes in defense-related enzymes and phenolics in resistant and susceptible common wheat cultivars under aphid stress
Changes in defense-related enzymes and phenolics in resistant and susceptible common wheat cultivars under aphid stress
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蚜虫胁迫下抗、感普通小麦品种防御相关酶和酚类物质的变化
DOI：
10.1007/s11738-021-03207-3
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发表时间：
2021-01
影响因子：
2.6
通讯作者：
Suqin Zhang
中科院分区：
文献类型：
--
作者：
Yi Xu;Hao Guo;Guangdong Geng;Qingqin Zhang;Suqin Zhang
禾谷缢管蚜Rhopalosiphum padiL.是粮食作物上的严重害虫，通过直接取食损害和病毒传播降低品质和产量。为了解小麦抗蚜机制，研究了两个小麦品种在蚜虫胁迫下生理生化指标的变化。在蚜虫取食过程中，抗病品种W 0923的苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）、酪氨酸解氨酶（TAL）、Ca 2 +-ATPase和Mg 2 +-ATPase的活性均显著高于感病品种GN 21。然而，这些酶活性在GN 21中先升高后降低。W 0923的多酚氧化酶（PPO）活性比GN 21的维持时间长。与对照相比，2个品种的总酚含量在第15天显著增加，到第30天W 0923的含量显著高于GN 21。在未处理的对照条件下，W 0923的POD、PPO、PAL、TAL和Ca ~（2+）-ATPase活性及酚类物质含量均高于GN 21。Ca ~（2+）-ATPase和PAL活性与POD、PPO和Mg ~（2+）-ATPase活性呈正相关。Ca ~（2+）-ATPase和PAL可能是评价蚜虫抗性的重要生化指标。与GN 21相比，W 0923具有较强的保持酶活性和合成酚类和单宁的能力，有助于抗蚜。
The cereal aphidsRhopalosiphum padiL. are serious pests on grain crops, reducing the quality and yield by direct feeding damage and virus transmission. The changes in the physiological and biochemical parameters of two wheat cultivars under aphid stress were investigated to understand aphid-resistance mechanisms. The activity levels of phenylalanine ammonia-lyase (PAL), peroxidase (POD), tyrosine ammonia-lyase (TAL), Ca2+-ATPase and Mg2+-ATPase in resistant cultivar W0923 increased during aphid feeding, and most of them were significantly higher than those in the susceptible cultivar GN21. However, these enzyme activities increased and then decreased in GN21. The polyphenol oxidase (PPO) activity in W0923 was maintained longer than in GN21. The total phenol contents of the two cultivars exhibited significant increases on the 15th day compared with the untreated controls, and the content in W0923 was significantly higher than in GN21 by the 30th day. Most of the POD, PPO, PAL, TAL and Ca2+-ATPase activities and phenolic metabolite contents were higher in W0923 than in GN21 under the untreated control conditions. The Ca2+-ATPase and PAL activities positively correlated with POD, PPO and Mg2+-ATPase activities. Ca2+-ATPase and PAL may be key biochemical markers for evaluating aphid resistance. W0923 had a strong ability to maintain higher enzyme activities and synthesize more phenols and tannins than GN21, which contributed to aphid resistance.
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影响因子：
4.8
作者：
D. Stokes;F. Delavoie;W. Rice;P. Champeil;D. McIntosh;J. Lacapere
通讯作者：
D. Stokes;F. Delavoie;W. Rice;P. Champeil;D. McIntosh;J. Lacapere
影响因子：
2.1
作者：
G. J. Liu;Donald K. Martin;R. Gardner;P. Ryan
通讯作者：
G. J. Liu;Donald K. Martin;R. Gardner;P. Ryan
影响因子：
7
作者：
Jin-song Huang;Yixiang Zhang;Li Jiang;Zhi-fang Yu
通讯作者：
Jin-song Huang;Yixiang Zhang;Li Jiang;Zhi-fang Yu
影响因子：
1.9
作者：
K. C. Jones;J. Klocke
通讯作者：
K. C. Jones;J. Klocke
DOI：
10.1016/j.scitotenv.2019.04.143
发表时间：
2019-07
期刊：
The Science of the total environment
影响因子：
--
作者：
Kai Zhang;Qiong Pan;Deying Yu;Liming Wang;Zhenzhen Liu;Xue Li;Xiyang Liu
通讯作者：
Kai Zhang;Qiong Pan;Deying Yu;Liming Wang;Zhenzhen Liu;Xue Li;Xiyang Liu