Effect of phenylephrine on pyruvate dehydrogenase in fasting rat livers.
Effect of phenylephrine on pyruvate dehydrogenase in fasting rat livers.
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去氧肾上腺素对空腹大鼠肝脏丙酮酸脱氢酶的影响。
DOI：
10.1111/j.1432-1033.1991.tb15798.x
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
LaNoue,KF
中科院分区：
文献类型：
--
作者：
Sterniczuk,A;Hreniuk,S;ScadutoJr,RC;LaNoue,KF
Previous estimates of flux through the pyruvate‐dehydrogenase complex were made by measuring14CO2generated from oxidation of [1‐14C]pyruvate, assuming a 1:1 stoichiometry. However, this method fails to discriminate between14CO2produced from pyruvate dehydrogenase and14CO2generated from phospho‐enolpyruvate carboxykinase and citric‐acid‐cycle dehydrogenases. While some previous reports have attempted to correct for the additional14CO2production by comparing14CO2generated by [1‐14C]pyruvate with [2‐14C]pyruvate or [3‐14C]pyruvate, the estimates are flawed by failure to determine the radioactivity and distribution of the14C label in the oxalacetate pool. The present method circumvents these problems by utilizing [1,4‐14C]succinate to radiolabel the oxalacetate pool and by directly measuring the specific radioactivity of malate. The results demonstrate that flux through the pyruvate‐dehydrogenase complex is negligible compared to the other reactions which generate14CO2from [1‐14C]lactate in the fasted state. Phenylephrine did not significantly alter this result in the fasted state. However,14CO2production via the pyruvate‐dehydrogenase complex is large (∼ 11.5 nmol · min−1· mg mitochondrial protein−1) compared to14CO2production via phosphoenolpyruvate carboxykinase and citric‐acid‐cycle dehydrogenases (∼ 6.4 nmol · min−1· mg−1) when the pyruvate‐dehydrogenase complex is activated, in the fed state with 1 mM dichloroacetate.
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胰高血糖素和其他 Ca2 动员激素对苹果酸天冬氨酸循环的作用位点。
影响因子：
3.9
作者：
T. Strzelecki;D. Strzelecka;C. Koch;K. Lanoue
通讯作者：
K. Lanoue
灌注大鼠肝脏中丙酮酸脱氢酶复合物的调节：线粒体单羧酸易位子的作用。
影响因子：
3.9
作者：
Patel,TB;Debuysere,MS;Scholz,R;Olson,MS
通讯作者：
Olson,MS
灌注肝脏中Ca2()相关的糖异生控制机制。
DOI：
10.1111/j.1432-1033.1991.tb15797.x
发表时间：
1991
期刊：
European journal of biochemistry
影响因子：
--
作者：
Sterniczuk,A;Hreniuk,S;ScadutoJr,RC;LaNoue,KF
通讯作者：
LaNoue,KF
[4] 大鼠肝脏灌注
DOI：
--
发表时间：
1975
期刊：
影响因子：
--
作者：
J. Exton
通讯作者：
J. Exton
脂肪酸对灌注大鼠肝脏丙酮酸脱氢酶调节的影响。
DOI：
--
发表时间：
1978
期刊：
European Journal of Biochemistry
影响因子：
--
作者：
R. Scholz;M. Olson;A. Schwab;U. Schwabe;C. Noell;W. Braun
通讯作者：
W. Braun