The effect of fatty acids on the regulation of pyruvate dehydrogenase in perfused rat liver.
The effect of fatty acids on the regulation of pyruvate dehydrogenase in perfused rat liver.
复制标题
脂肪酸对灌注大鼠肝脏丙酮酸脱氢酶调节的影响。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
W. Braun
中科院分区：
文献类型：
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作者：
R. Scholz;M. Olson;A. Schwab;U. Schwabe;C. Noell;W. Braun
研究了脂肪酸对大鼠灌流肝脏丙酮酸脱羧速率的影响。从输注的[1- 14 C]丙酮酸产生的14 CO2被用作通过丙酮酸脱氢酶反应的通量的监测器。使用动力学分析对其他脱羧反应进行校正。脂肪酸在灌注液中丙酮酸浓度低于1 mM时，（辛酸盐或油酸盐）输注引起丙酮酸脱羧的刺激在0.05 mM丙酮酸盐浓度下可达3倍），但在接近5 mM丙酮酸盐浓度下，速率降低至对照速率的三分之一。在低丙酮酸浓度下，羟基丁酸也引起丙酮酸脱羧的显著刺激。这些数据表明，脂肪酸刺激通过丙酮酸脱氢酶反应在灌注的肝脏在低（限制）丙酮酸浓度的流量的机制涉及到丙酮酸运输到线粒体室由于与乙酰乙酸的交换加速。此外，有人提出，在丙酮酸浓度接近体内条件下，生酮和丙酮酸氧化调节之间存在关系。
The effect of fatty acids on the rate of pyruvate decarboxylation was studied in perfused livers from fed rats. The production of 14CO2 from infused [1-14C]pyruvate was employed as a monitor of the flux through the pyruvate dehydrogenase reaction. A correction for other decarboxylation reactions was made using kinetic analyses. Fatty acid (octanoate or oleate) infusion caused a stimulation of pyruvate decarboxylation at pyruvate concentrations in the perfusate below 1 mM (up to 3-fold at 0.05 mM pyruvate) but decreased the rate to one-third of control rates at pyruvate concentrations near 5 mM. These effects were half-maximal at fatty acid concentrations below 0.1 mM. Infusion of 3-hydroxybutyrate also caused a marked stimulation of pyruvate decarboxylation at low pyruvate concentrations. The data suggest that the mechanism by which fatty acids stimulate the flux through the pyruvate dehydrogenase reaction in perfused liver at low (limiting) pyruvate concentrations involves an acceleration of pyruvate transport into the mitochondrial compartment due to an exchange with acetoacetate. Furthermore, it is proposed that a relationship exists between ketogenesis and the regulation of pyruvate oxidation at pyruvate concentrations approximating conditions in vivo.
脑丙酮酸脱氢酶活性：通过磷酸化-去磷酸化调节。
影响因子：
2.9
作者：
Morgan,DG;Routtenberg,A
通讯作者：
Routtenberg,A