In vitro study of the effects of Angiostrongylus cantonensis larvae extracts on apoptosis and dysfunction in the blood-brain barrier (BBB).
In vitro study of the effects of Angiostrongylus cantonensis larvae extracts on apoptosis and dysfunction in the blood-brain barrier (BBB).
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DOI：
10.1371/journal.pone.0032161
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Tan F
中科院分区：
文献类型：
--
作者：
Hu X;Li JH;Lan L;Wu FF;Zhang EP;Song ZM;Huang HC;Luo FJ;Pan CW;Tan F
据推测，广州管圆线虫感染中的血脑屏障（BBB）功能障碍可能是由于宿主血脑屏障细胞的凋亡所致。在此，我们通过几种方法对这一假设进行了评估，这些方法均基于一个由原代培养的脑微血管内皮细胞（BMECs）和脑星形胶质细胞（BACs）组成的体外小鼠血脑屏障模型。在本研究中，4小时的渗滤和辣根过氧化物酶（HRP）通透性实验表明，广州管圆线虫幼虫提取物可增加血脑屏障的通透性。通过TUNEL法和膜联蛋白 - V - FITC/碘化丙啶（PI）双染色监测暴露于幼虫提取物后BMECs和BACs中的细胞凋亡情况。发现广州管圆线虫幼虫提取物可诱导BMECs和BACs两者的细胞凋亡。因此，我们得出结论，细胞凋亡的诱导可能参与了广州管圆线虫病期间所观察到的血脑屏障功能障碍。对广州管圆线虫如何诱导细胞凋亡的深入理解可能会为这种寄生虫病的治疗或预防带来新的方法。
It has been hypothesized that blood-brain barrier (BBB) dysfunction in Angiostrongylus cantonensis infection might be due to the apoptosis of the hosts' BBB cells. Here, we evaluated this hypothesis through several methods, all based on an in vitro mouse BBB model consisting of primary culture brain microvascular endothelial cells (BMECs) and brain astrocytic cells (BACs). In the present study, a four-hour percolation and HRP permeability experiment showed that A. cantonensis larvae extracts can increase the permeability of the BBB. Apoptosis among BMECs and BACs after exposure to larvae extracts was monitored by TUNEL and annexin-V-FITC/PI double staining. A. cantonensis larvae extracts were found to induce apoptosis in both BMECs and BACs. For this reason, we concluded that the induction of apoptosis might participate in the BBB dysfunction observed during angiostrongyliasis. Improved fundamental understanding of how A. cantonensis induces apoptosis may lead to new approaches to the treatment or prevention of this parasitic disease.
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影响因子：
9.9
作者：
Brown, HC;Chau, TTH;Turner, GDH
通讯作者：
Turner, GDH
影响因子：
4.2
作者：
Isobe, I;Watanabe, T;Kato, T
通讯作者：
Kato, T
DOI：
10.1128/cdli.5.4.452-455.1998
发表时间：
1998-07-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Dorta-Contreras, A;Reiber, H
通讯作者：
Reiber, H
影响因子：
3.3
作者：
Kolb, SA;Lahrtz, F;Fontana, A
通讯作者：
Fontana, A
影响因子：
1.5
作者：
Stiles, JK;Whittaker, J;Bond, VC
通讯作者：
Bond, VC