Functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP
Functional interaction of low-homology FRPs from different cyanobacteria with Synechocystis OCP
复制标题
不同蓝藻低同源性 FRP 与集胞藻 OCP 的功能相互作用
DOI：
10.1101/247882
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
N. Sluchanko
中科院分区：
文献类型：
--
作者：
Y. B. Slonimskiy;E. Maksimov;E. Lukashev;M. Moldenhauer;C. Jeffries;D. Svergun;T. Friedrich;N. Sluchanko
光合作用需要在高效的光收集和光损伤保护之间取得平衡。蓝藻的光保护系统唯一依赖于光活性橙色类胡萝卜素蛋白(OCP)的功能，该蛋白在强光下提供捕光天线复合体-藻胆体的荧光猝灭。最近发现的荧光恢复蛋白(FRP)与光激活的OCP结合，并加速其松弛进入基础形式，完成调节环。FRP功能的分子机制在很大程度上存在争议。此外，由于现有的知识主要是通过研究聚球藻蛋白获得的，因此FRP机制的跨物种保守性仍未被探索。除了系统发育分析外，我们还通过与集胞藻FRP(SynFRP)的比较，对两个同源性较低的FRP进行了详细的结构和功能分析。在溶液中采用相似的二聚体构象并保持SynFRP对各种OCP变体的结合偏好的同时，低同源性的FRP显示出明显的结合化学计量学和这种功能相互作用的不同突出特征。通过提供普遍了解FRP机制的线索，我们的结果也为即将到来的结构调查奠定了基础，这些研究是阐明FRP依赖的调控机制所必需的。
Photosynthesis requires a balance between efficient light harvesting and protection against photodamage. The cyanobacterial photoprotection system uniquely relies on the functioning of the photoactive orange carotenoid protein (OCP) that under intense illumination provides fluorescence quenching of the light-harvesting antenna complexes, phycobilisomes. The recently identified fluorescence recovery protein (FRP) binds to the photoactivated OCP and accelerates its relaxation into the basal form, completing the regulatory circle. The molecular mechanism of FRP functioning is largely controversial. Moreover, since the available knowledge has mainly been gained from studying Synechocystis proteins, the cross-species conservation of the FRP mechanism remains unexplored. Besides phylogenetic analysis, we performed a detailed structural-functional analysis of two selected low-homology FRPs by comparing them with Synechocystis FRP (SynFRP). While adopting similar dimeric conformations in solution and preserving binding preferences of SynFRP toward various OCP variants, the low-homology FRPs demonstrated distinct binding stoichiometries and differentially accentuated features of this functional interaction. By providing clues to understand the FRP mechanism universally, our results also establish foundations for upcoming structural investigations necessary to elucidate the FRP-dependent regulatory mechanism.
影响因子：
2.9
作者：
Lu Y;Liu H;Saer R;Li VL;Zhang H;Shi L;Goodson C;Gross ML;Blankenship RE
通讯作者：
Blankenship RE
影响因子：
6.1
作者：
Franke D;Petoukhov MV;Konarev PV;Panjkovich A;Tuukkanen A;Mertens HDT;Kikhney AG;Hajizadeh NR;Franklin JM;Jeffries CM;Svergun DI
通讯作者：
Svergun DI