CD8 T cells producing IL-17 and IFN-gamma initiate the innate immune response required for responses to antigen skin challenge.
CD8 T cells producing IL-17 and IFN-gamma initiate the innate immune response required for responses to antigen skin challenge.
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产生 IL-17 和 IFN-gamma 的 CD8 T 细胞启动应对抗原皮肤挑战所需的先天免疫反应。
DOI：
10.4049/jimmunol.0802830
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发表时间：
2009-05-15
影响因子：
4.4
通讯作者：
Fairchild, Robert L.
中科院分区：
文献类型：
--
作者：
Kish, Danielle D.;Li, Xiaoxia;Fairchild, Robert L.
响应抗原攻击而将效应 CD8 T 细胞招募到皮肤中需要事先 CXCL1/KC 引导的中性粒细胞浸润。研究了诱导 CXCL1 产生的机制以及在皮肤中引发抗原特异性反应期间中性粒细胞-CD8 T 细胞相互作用的动态。抗原致敏小鼠皮肤激发部位的 CXCL1 和 CXCL2/MIP-2 在抗原激发后 3-6 小时内产生，水平比非致敏小鼠高 10 倍。在致敏小鼠的攻击部位，这种产生在攻击后 6-9 小时下降，接近非致敏小鼠皮肤攻击部位观察到的水平，但在攻击后 12 小时上升到第二个峰值。致敏动物皮肤刺激后 3-6 小时，早期中性粒细胞趋化剂的产生增加，需要 IFN-γ 和 IL-17，它们是由不同的抗原引发的 CD8 T 细胞群响应抗原刺激而产生的。尽管是由抗原引发的 CD8 T 细胞诱导的，但早期 CXCL1 和 CXCL2 的产生伴随着中性粒细胞，而不是 CD8 T 细胞浸润到皮肤抗原攻击部位。直到攻击后 18-24 小时才观察到 CD8 T 细胞浸润到攻击部位。这些结果证明了抗原特异性和先天免疫成分之间存在一系列复杂的早期相互作用，这些成分调节中性粒细胞的顺序浸润，然后调节效应 T 细胞进入皮肤以介导免疫反应。
Effector CD8 T cell recruitment into the skin in response to antigen challenge requires prior CXCL1/KC-directed neutrophil infiltration. Mechanisms inducing CXCL1 production and the dynamics of neutrophil-CD8 T cell interactions during elicitation of antigen-specific responses in the skin were investigated. CXCL1 and CXCL2/MIP-2 were produced within 3–6 hours of antigen challenge at 10-fold higher levels in skin challenge sites of antigen-sensitized vs. non-sensitized mice. In the challenge sites of sensitized mice this production decreased at 6–9 hours post-challenge to near the levels observed in skin challenge sites of non-sensitized mice but rose to a second peak 12 hours after challenge. The elevated early neutrophil chemoattractant production at 3–6 hours after skin challenge of sensitized animals required both IFN-γ and IL-17, produced by distinct populations of antigen-primed CD8 T cells in response to antigen challenge. Although induced by the antigen-primed CD8 T cells, the early CXCL1 and CXCL2 production was accompanied by neutrophil but not CD8 T cell infiltration into the skin antigen challenge site. Infiltration of the CD8 T cells into the challenge site was not observed until 18–24 hours after challenge. These results demonstrate an intricate series of early interactions between antigen-specific and innate immune components that regulate the sequential infiltration of neutrophils and then effector T cells into the skin to mediate an immune response.
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DOI：
10.1084/jem.166.6.1654
发表时间：
1987-12-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Macatonia SE;Knight SC;Edwards AJ;Griffiths S;Fryer P
通讯作者：
Fryer P
影响因子：
5.5
作者：
Engeman, T;Gorbachev, AV;Fairchid, RL
通讯作者：
Fairchid, RL
影响因子：
4.4
作者：
Gorbachev, AV;Fairchild, RL
通讯作者：
Fairchild, RL
影响因子：
4.4
作者：
Gorbachev, AV;Heeger, PS;Fairchild, RL
通讯作者：
Fairchild, RL
影响因子：
4.4
作者：
Cruz, Andrea;Khader, Shabaana A.;Castro, Antonio G.
通讯作者：
Castro, Antonio G.