On the Cutting Edge of Translational Research.
On the Cutting Edge of Translational Research.
复制标题
论转化研究的前沿。
DOI：
10.1093/clinchem/hvaa273
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发表时间：
2021
影响因子：
9.3
通讯作者：
Angel,PeggiM
中科院分区：
文献类型：
--
作者：
Angel,PeggiM
在癌症诊断和治疗管理中，人们不断寻找可以预测患者分层和反应的新分子因子。这些研究主要在人体组织中进行，目的是获取临床使用的人类疾病过程的基本信息。这类实验室研究的目标是将获得的信息迅速地转移到临床应用中，被称为转化研究。转化研究的早期定义是将获得的知识在实验室工作台和临床应用之间的任何一个方向上转移的能力（1）。然而，实验室研究和临床应用的工具通常是分开的类别，特别是关于对人类和人体组织的适用性。如果仅关注质谱研究工具，则主要问题将是使用质谱的方法通常是耗时的，并且需要大量的专业知识来获取和应用数据信息以用于临床使用。质谱在临床实验室中的移动通常是单向的，多年来从实验室转移到临床应用。需要真正的平移质谱工具，可以在临床环境中报告信息，就像在基础实验室研究环境中一样容易。iKnife（2）是一种质谱分析工具，在临床和研究实验室之间移动数据方面表现出很大的希望。在最近发表在Cell上的一篇文章中，Koundouros et al. (3)采用了一种独特的方法，使用iKnife，一种已经在外科手术室使用的工具，用于基础实验室研究。iKnife将电外科切割与快速蒸发电离质谱法相结合，以接近真实的时间（1-2 s）评估术中肿瘤边缘和活检特征（2）。通过质谱法直接分析由电外科切割发出的羽流，并与包含以下的光谱库进行比较：
In cancer diagnosis and therapeutic management, novel molecular factors are continually being sought that can predict patient stratification and response. These studies are performed primarily on human tissues with the objective of acquiring basic information on human disease processes toward clinical use. This type of laboratory research, with the goal of moving the acquired information rapidly toward clinical application, is regarded as translational research.An early definition of translational research was the ability to move acquired knowledge in either direction between laboratory bench and clinical application (1). However, the tools of laboratory research and clinical application have typically been in separate categories, especially regarding applicability to human beings and human tissues. If one focuses only on mass spectrometry research tools, a main problem would be that the approaches using mass spectrometry are typically time consuming and require substantial expertise to acquire and apply data information for clinical use. Movement of mass spectrometry in the clinical laboratory has typically been one way, moving from bench to clinical application over years. Truly translational mass spectrometry tools are needed that can report information just as easily in the clinical setting as in the basic laboratory research setting. One mass spectrometry tool showing great promise in moving data between clinic and research laboratory and back again is the iKnife (2). In a recent article in Cell, Koundouros et al.(3) took a unique approach using the iKnife, a tool already in the surgical theater, for basic laboratory research. The iKnife combines electrosurgical dissection with rapid evaporative ionization mass spectrometry to assess intraoperative tumor margins and signatures from biopsies in near real time (1–2 s)(2). The plume emitted by electrosurgical dissection is directly analyzed by mass spectrometry and compared with spectral libraries that contain
影响因子：
9.3
作者：
Ifa DR;Eberlin LS
通讯作者：
Eberlin LS
iKnife：快速蒸发电离质谱的开发和临床应用
DOI：
--
发表时间：
2019
期刊：
The Handbook of Metabolic Phenotyping
影响因子：
--
作者：
B. Vaqas;Simon J. S. Cameron;J. Alexander;K. O’Neill;J. Kinross;Z. Takáts
通讯作者：
Z. Takáts