Biochip Synthesis and Dynamic Error Recovery for Sample Preparation Using Digital Microfluidics
Biochip Synthesis and Dynamic Error Recovery for Sample Preparation Using Digital Microfluidics
复制标题
使用数字微流体进行样品制备的生物芯片合成和动态误差恢复
DOI：
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发表时间：
2014
影响因子：
2.9
通讯作者：
K. Chakrabarty
中科院分区：
文献类型：
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作者：
Yi;Tsung;K. Chakrabarty
数字微流控生物芯片的最新进展为小型化实验室带来了光明的未来，具有高灵敏度和可重构性的相关优势。由于样品制备在生化应用的测定和实验室中发挥着重要的前端作用，并且大部分分析时间与样品收集、运输和制备相关，因此最大限度地减少生物测定中这一关键步骤所需的时间非常重要。此外，确保中间步骤的正确性并在样品制备期间有效地从错误中恢复也至关重要。我们描述了一个优化算法和相关的芯片设计方法的样品制备，包括建筑综合和布局综合。我们还提出了在样品制备过程中使用的第一个动态错误恢复程序。所提出的算法在现实生活中的生物化学应用和合成测试用例进行评估，以证明其有效性和效率。与现有的工作相比，该算法可以实现高达50%的样品制备时间减少，优化的芯片布局可以实现样品制备时间减少40%以上。
Recent advances in digital microfluidic biochips have led to a promising future for miniaturized laboratories, with the associated advantages of high sensitivity and reconfigurability. Since sample preparation plays an important front-end role in assays and laboratories in biochemical applications, and most of the analysis time is associated with sample collection, transportation, and preparation, it is important to minimize the time required for this key step in bioassays. Moreover, it is also critical to ensure the correctness of intermediate steps and recover from errors efficiently during sample preparation. We describe an optimization algorithm and the associated chip design method for sample preparation, including architectural synthesis and layout synthesis. We also present the first dynamic error recovery procedure for use during sample preparation. The proposed algorithm is evaluated on both real-life biochemical applications and synthetic test cases to demonstrate its effectiveness and efficiency. Compared to prior work, the proposed algorithm can achieve up to 50% reduction in sample preparation time, and the optimized chip layout can achieve over 40% reduction in sample preparation time.
影响因子：
6.1
作者：
Sista R;Hua Z;Thwar P;Sudarsan A;Srinivasan V;Eckhardt A;Pollack M;Pamula V
通讯作者：
Pamula V