Development of a digital microfluidic platform for point of care testing.
Development of a digital microfluidic platform for point of care testing.
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DOI：
10.1039/b814922d
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发表时间：
2008-12
期刊：
影响因子：
6.1
通讯作者：
Pamula V
中科院分区：
文献类型：
--
作者：
Sista R;Hua Z;Thwar P;Sudarsan A;Srinivasan V;Eckhardt A;Pollack M;Pamula V
在卫生保健管理中，护理点检测在提高临床效果方面发挥着越来越重要的作用。护理点设备的显著特点是快速结果，集成样品制备和处理，小样本量，便携性，多功能和低成本。在本文中，我们利用基于电润湿的数字微流控平台展示了其中的一些显著特征。我们使用全血样本，在不到8分钟的时间内，在数字微流控盒上演示了基于磁珠的免疫测定（心脏肌钙蛋白I）的性能。使用相同的微流体盒，通过在两个热区之间穿梭液滴，在12分钟内完成40个周期的实时聚合酶链反应。我们进一步证明，在同一个药筒上，使用磁珠对细菌和真菌传染病病原体（耐甲氧西林金黄色葡萄球菌和白色念珠菌）和人类基因组DNA进行样品制备的能力。除了快速的结果和集成的样品制备，基于电润湿的数字微流体仪器是高度便携的，因为流体泵送是电子执行的。本文介绍的所有数字微流控芯片都是利用大规模生产技术在印刷电路板上制造的，以保持芯片的低成本。由于数字微流体提供的模块化和可扩展性，多功能测试能力，例如免疫分析，DNA扩增和酶分析之间的组合，可以以相对较低的成本带到护理点，因为单个芯片可以在软件中配置用于护理路径所需的不同分析。
Point of care testing is playing an increasingly important role in improving the clinical outcome in health care management. The salient features of a point of care device are quick results, integrated sample preparation and processing, small sample volumes, portability, multifunctionality and low cost. In this paper, we demonstrate some of these salient features utilizing an electrowetting-based Digital Microfluidic platform. We demonstrate the performance of magnetic bead-based immunoassays (cardiac troponin I) on a digital microfluidic cartridge in less than 8 minutes using whole blood samples. Using the same microfluidic cartridge, a 40-cycle real-time polymerase chain reaction was performed within 12 minutes by shuttling a droplet between two thermal zones. We further demonstrate, on the same cartridge, the capability to perform sample preparation for bacterial and fungal infectious disease pathogens (methicillin-resistance Staphylococcus aureus and Candida albicans) and for human genomic DNA using magnetic beads. In addition to rapid results and integrated sample preparation, electrowetting-based digital microfluidic instruments are highly portable because fluid pumping is performed electronically. All the digital microfluidic chips presented here were fabricated on printed circuit boards utilizing mass production techniques that keep the cost of the chip low. Due to the modularity and scalability afforded by digital microfluidics, multifunctional testing capability, such as combinations within and between immunoassays, DNA amplification, and enzymatic assays, can be brought to the point of care at a relatively low cost because a single chip can be configured in software for different assays required along the path of care.
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影响因子：
2.7
作者：
Cho, SK;Moon, HJ;Kim, CJ
通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
Wixforth, A
影响因子：
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作者：
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通讯作者：
Pamula VK
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