Identification of wild soybean miRNAs and their target genes responsive to aluminum stress.
Identification of wild soybean miRNAs and their target genes responsive to aluminum stress.
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DOI：
10.1186/1471-2229-12-182
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发表时间：
2012-10-05
影响因子：
5.3
通讯作者：
Nian H
中科院分区：
文献类型：
--
作者：
Zeng QY;Yang CY;Ma QB;Li XP;Dong WW;Nian H
microRNAs（miRNAs）在植物的发育和逆境应答中起着重要的调控作用。野生大豆（Glycine soja）经历了长期的自然选择，可能已经进化出特殊的机制来适应逆境条件。然而，关于野生大豆中的miRNAs，特别是铝胁迫下的miRNAs的研究却很少。从铝处理和无铝处理的根中构建了两个小RNA文库和两个降解组文库。大豆幼苗对于miRNA鉴定，在Al处理的和无Al的小RNA文库中分别产生总共7，287，655和7，035，914个干净读段，并且鉴定了97个已知的miRNA和31个新的miRNA。此外，还发现了49条已知的p3或p5链的miRNAs，其中30条miRNAs的表达与铝胁迫有关。通过degradome测序，共鉴定出86个已知miRNAs的靶基因，其中5个基因是本研究中获得的新miRNAs的靶基因。基因本体论（GO）对靶基因转录本的注释表明，有52个靶基因被保守的miRNA家族切割，可能在转录调控中发挥作用。此外，一些基因，如生长素反应因子（ARF），含有结构域的抗病蛋白（NB-ARC），富含亮氨酸的重复和toll/白细胞介素-1受体样蛋白（LRR-TIR）结构域蛋白，阳离子转运ATP酶，Myb转录因子，和无顶端分生组织（NAM）蛋白，已知是响应于应力，被发现在铝胁迫条件下被切割。在野生大豆中检测到大量的miRNAs及其靶基因。其中一些对生物胁迫和非生物胁迫有反应的酶受到铝胁迫的调控。这些发现为理解miRNAs在铝耐受中的作用提供了有价值的信息。
MicroRNAs (miRNAs) play important regulatory roles in development and stress response in plants. Wild soybean (Glycine soja) has undergone long-term natural selection and may have evolved special mechanisms to survive stress conditions as a result. However, little information about miRNAs especially miRNAs responsive to aluminum (Al) stress is available in wild soybean. Two small RNA libraries and two degradome libraries were constructed from the roots of Al-treated and Al-free G. soja seedlings. For miRNA identification, a total of 7,287,655 and 7,035,914 clean reads in Al-treated and Al-free small RNAs libraries, respectively, were generated, and 97 known miRNAs and 31 novel miRNAs were identified. In addition, 49 p3 or p5 strands of known miRNAs were found. Among all the identified miRNAs, the expressions of 30 miRNAs were responsive to Al stress. Through degradome sequencing, 86 genes were identified as targets of the known miRNAs and five genes were found to be the targets of the novel miRNAs obtained in this study. Gene ontology (GO) annotations of target transcripts indicated that 52 target genes cleaved by conserved miRNA families might play roles in the regulation of transcription. Additionally, some genes, such as those for the auxin response factor (ARF), domain-containing disease resistance protein (NB-ARC), leucine-rich repeat and toll/interleukin-1 receptor-like protein (LRR-TIR) domain protein, cation transporting ATPase, Myb transcription factors, and the no apical meristem (NAM) protein, that are known to be responsive to stress, were found to be cleaved under Al stress conditions. A number of miRNAs and their targets were detected in wild soybean. Some of them that were responsive to biotic and abiotic stresses were regulated by Al stress. These findings provide valuable information to understand the function of miRNAs in Al tolerance.
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影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC
影响因子：
2.6
作者：
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通讯作者：
HYMOWITZ, T
影响因子：
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作者：
Addo-Quaye, Charles;Eshoo, Tifani W.;Axtell, Michael J.
通讯作者：
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影响因子：
6.9
作者：
Ding Y;Chen Z;Zhu C
通讯作者：
Zhu C
影响因子：
7.2
作者：
Huang, Chao-Feng;Yamaji, Naoki;Ma, Jian Feng
通讯作者：
Ma, Jian Feng