Anti-apoptotic effect of claudin-1 in tamoxifen-treated human breast cancer MCF-7 cells.
Anti-apoptotic effect of claudin-1 in tamoxifen-treated human breast cancer MCF-7 cells.
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DOI：
10.1186/1471-2407-10-548
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发表时间：
2010-10-12
期刊：
影响因子：
3.8
通讯作者：
Kijima H
中科院分区：
文献类型：
--
作者：
Akasaka H;Sato F;Morohashi S;Wu Y;Liu Y;Kondo J;Odagiri H;Hakamada K;Kijima H
Claudin-1是一种紧密连接的膜蛋白，与多种癌症的发生有关。然而，claudin-1在癌细胞中表达的意义还不清楚。在这里，我们首次展示了claudin-1在人乳腺癌MCF-7细胞中的抗凋亡作用。用或不用他莫昔芬、针对claudin-1的siRNA或他莫昔芬和claudin-1 siRNA处理人乳腺癌MCF-7和T47 D细胞。采用RT-PCR、Western blotting或免疫荧光染色等方法对样品进行分析。claudin-1的表达在他莫昔芬处理的MCF-7细胞中上调，而claudin-1的表达在他莫昔芬处理的T47 D细胞中没有改变。在MCF-7细胞中，通过siRNA敲低claudin-1增加了聚（ADP-核糖）聚合酶（PARP）的量，而与他莫昔芬治疗无关，但不是T47 D细胞。在MCF-7细胞的细胞膜中，他莫昔芬处理增加了claudin-1的量，但减少了β-catenin的量。Claudin-1 siRNA可增加MCF-7细胞胞质中E-钙粘蛋白的量以及细胞膜中β-连环蛋白的量。这些结果表明，claudin-1具有抗凋亡作用，并参与调节MCF-7细胞中β-catenin和E-cadherin的表达和亚细胞定位，但不参与T47 D细胞中的表达和亚细胞定位。
Claudin-1 is a membrane protein of tight junctions, and is associated with the development of various cancers. However, the significance of claudin-1 expression in cancer cells is not well understood. Here, we showed for the first time the anti-apoptotic effect of claudin-1 in human breast cancer MCF-7 cells. Human breast cancer MCF-7 and T47 D cells were treated with or without tamoxifen, siRNA against claudin-1, or tamoxifen and claudin-1 siRNA. The samples were analyzed by RT-PCR, Western blotting or immunofluorescent staining. The expression of claudin-1 was upregulated in tamoxifen-treated MCF-7 cells, whereas the expression of claudin-1 was not altered in tamoxifen-treated T47 D cells. Knockdown of claudin-1 by siRNA increased the amount of poly (ADP-ribose) polymerase (PARP) regardless of tamoxifen treatment in MCF-7 cells, but not T47 D cells. In the cell membranes of the MCF-7 cells, tamoxifen treatment increased the amount of claudin-1, but decreased the amount of β-catenin. Claudin-1 siRNA increased the amount of E-cadherin in the cytoplasm of the MCF-7 cells as well as the amount of β-catenin in their cell membranes. These results indicate that claudin-1 has anti-apoptotic effects, and is involved in the regulation of the expression and subcellular localization of β-catenin and E-cadherin in MCF-7, but not T47 D cells.
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影响因子：
254.7
作者：
Jemal, Ahmedin;Siegel, Rebecca;Thun, Michael J.
通讯作者：
Thun, Michael J.
影响因子：
4.3
作者：
He, Yu-Jun;Liu, Bao-Hua;He, Yu-Hong
通讯作者：
He, Yu-Hong
影响因子：
9.7
作者：
Sandhya, T.;Mishra, K. P.
通讯作者：
Mishra, K. P.
影响因子：
--
作者：
Sjöström, J;Bergh, J
通讯作者：
Bergh, J
单个基因产物Claudin -1或-2，在成纤维细胞中重构紧密结合的连接链和新兵。
影响因子：
7.8
作者：
Furuse, M;Sasaki, H;Fujimoto, K;Tsukita, S
通讯作者：
Tsukita, S