mRNA and micro-RNA expression analysis in laser-capture microdissected prostate biopsies: valuable tool for risk assessment and prevention trials.
mRNA and micro-RNA expression analysis in laser-capture microdissected prostate biopsies: valuable tool for risk assessment and prevention trials.
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DOI：
10.1016/j.yexmp.2009.10.005
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发表时间：
2010-02
影响因子：
3.6
通讯作者：
Gann, Peter H.
中科院分区：
文献类型：
--
作者：
Nonn, Larisa;Vaishnav, Avani;Gallagher, Lindsay;Gann, Peter H.
前列腺癌（PCa）的诊断通常依赖于针吸活检，这些活检通常在福尔马林固定后保存在石蜡中，可能包含有价值的风险或预后信息。本研究的目的是确定与冷冻组织的金标准相比，激光捕获显微切割（LCM）福尔马林固定石蜡包埋存档前列腺活检组织中mRNA和miRNA表达分析的可行性。我们分析了间隔特异性和PCA相关基因在上皮和间质组织中的表达，这些组织是从三名患者的存档前列腺活检和冷冻根治性前列腺切除术标本中收集的。我们的研究结果显示，FFPE活检和冷冻组织之间具有良好的患者内一致性，具有适当的隔室特异性和PC相关表达。使用PCR阵列也证明了活检中mRNA和microRNA表达谱的潜力，其显示活检和冷冻组织之间的高度相关性，尽管FFPE标本中mRNA检测的灵敏度有限。这是第一项比较来自同一患者的活检组织和冷冻组织的RNA表达并检查来自前列腺活检的LCM收集组织中的miRNA表达的研究。通过仔细的技术和使用适当的对照，存档活检材料的RNA谱分析是非常可行的，显示出与冷冻组织的高度相关性。
Diagnosis of prostate cancer (PCa) typically relies on needle biopsies, which are routinely archived in paraffin after formalin fixation and may contain valuable risk or prognostic information. The objective of this study was to determine the feasibility of mRNA and miRNA expression analysis in laser-capture microdissected (LCM) formalin-fixed paraffin-embedded archived prostate biopsies compared to the gold standard of frozen tissue. We analyzed the expression of compartment-specific and PCa-related genes in epithelial and stromal tissues collected from paired sets of archived prostate biopsies and frozen radical prostatectomy specimens from three patients. Our results showed appropriate compartment-specific and PC-related expression with good within patient agreement between the FFPE-biopsies and the frozen tissue. The potential for both mRNA and microRNA expression profiling in the biopsies was also demonstrated using PCR arrays which showed high correlation between the biopsy and frozen tissue, notwithstanding sensitivity limitations for mRNA detection in the FFPE specimen. This is the first study to compare RNA expression from biopsy and frozen tissues from the same patient and to examine miRNA expression in LCM-collected tissue from prostate biopsies. With careful technique and use of appropriate controls, RNA profiling from archived biopsy material is quite feasible showing high correlation to frozen tissue.
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影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
影响因子：
2.8
作者：
Prueitt, Robyn L.;Yi, Ming;Hudson, Robert S.;Wallace, Tiffany A.;Howe, Tiffany M.;Yfantis, Harris G.;Lee, Dong. H.;Stephens, Robert M.;Liu, Chang-Gong;Calin, George A.;Croce, Carlo M.;Ambs, Stefan
通讯作者：
Ambs, Stefan
影响因子：
2.8
作者：
Gelmann, EP;Bowen, C;Bubendorf, L
通讯作者：
Bubendorf, L
影响因子：
2.1
作者：
Darson, MF;Pacelli, A;Bostwick, DG
通讯作者：
Bostwick, DG
影响因子：
4.5
作者：
Rogerson, Lynsey;Darby, Steve;Gnanapragasam, Vincent J.
通讯作者：
Gnanapragasam, Vincent J.