A cytoskeleton-associated RNA-binding protein binds to the untranslated regions of prolamine mRNA and to poly(A).
A cytoskeleton-associated RNA-binding protein binds to the untranslated regions of prolamine mRNA and to poly(A).
复制标题
细胞骨架相关的 RNA 结合蛋白与谷醇溶蛋白 mRNA 的非翻译区和聚腺苷酸 (poly(A)) 结合。
DOI：
10.1016/s0168-9452(99)00156-9
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发表时间：
2000
期刊：
影响因子：
5.2
通讯作者：
T. Okita
中科院分区：
文献类型：
--
作者：
R. Sami;D. Muench;T. Okita
在发育中的水稻胚乳中，脯氨酸mrna在广泛内质网（ER）系统上不是随机翻译的。相反，它们定位于脯胺蛋白体（PBs）的表面。最近的研究结果表明，脯胺多体不仅附着在内质网表面，而且附着在与该细胞器相关的细胞骨架上。为了揭示脯胺mrna在水稻胚乳细胞中定位的分子和细胞基础，我们获得了富集的细胞骨架- pb片段，并研究了聚(U)-Sepharose获得的片段的RNA结合活性。通过rna -蛋白UV交联实验，我们发现了一个与细胞骨架- pb富集部分相关的优势40 kda rna结合蛋白。这种活性与脯胺mRNA的3 ‘和5 ’非翻译区以及谷氨酸mRNA的3 '非翻译区结合，但不与它们的编码序列结合。结合活性很容易与聚腺苷酸竞争，表明结合识别位点富含a。
Prolamine mRNAs are not randomly translated on the extensive endoplasmic reticulum (ER) system in developing rice endosperm. Instead they are localized on the surface of the prolamine protein bodies (PBs). Recent findings suggest that prolamine polysomes are attached to the surface of the prolamine PBs not only to the ER but also to the cytoskeleton which is associated with this organelle. To uncover the molecular and cellular basis for the localization of prolamine mRNAs in the rice endosperm cells, we obtained a enriched cytoskeleton-PB fraction and studied the RNA binding activities associated from fractions obtained from poly(U)-Sepharose. Using a RNA–protein UV cross-linking assay we identified a dominant 40-kDa RNA-binding protein associated with the cytoskeleton-PB-enriched fraction. This activity binds to the 3′ and 5′ untranslated regions of prolamine mRNA and the 3′ untranslated region of glutelin mRNA but not to their coding sequences. Binding activity is readily competed by polyadenylic acid indicating that the binding recognition site is A-rich.
影响因子：
14.9
作者：
Ma, WJ;Chung, S;Furneaux, H
通讯作者：
Furneaux, H
DOI：
--
发表时间：
1993-10
期刊：
The Journal of Cell Biology
影响因子：
--
作者：
J. Wilhelm;R. Vale
通讯作者：
J. Wilhelm;R. Vale
Poly(A) 结合蛋白在叶绿体中作为信息特异性翻译因子发挥作用。
DOI：
10.1073/pnas.95.5.2238
发表时间：
1998
影响因子：
11.1
作者：
Yohn,CB;Cohen,A;Danon,A;Mayfield,SP
通讯作者：
Mayfield,SP