Chromosomal instability and phenotypic variation in a specific lineage derived from a synthetic allotetraploid wheat.
Chromosomal instability and phenotypic variation in a specific lineage derived from a synthetic allotetraploid wheat.
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DOI：
10.3389/fpls.2022.981234
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
新形成的植物异源多倍体通常存在减数分裂缺陷，导致染色体不稳定，表现为染色体数量和/或结构的变化。然而，并不是所有新生的异源多倍体都同样不稳定。小麦类群(山羊草/小麦)包含13个具有不同基因组类型的二倍体物种。这些物种中的许多可以被人工杂交，以产生可行但不育的种间/属间F1杂种，这种杂种可以在全基因组加倍后产生可育的合成异源四倍体小麦。与含有AADD和S*S*DD(S*指的是不同物种的S相关基因组)基因组组合的合成异源四倍体小麦相比，那些含有S*S*AA基因组的合成异源四倍体小麦明显更稳定。然而，S*S*AA基因组相对稳定性的稳健性尚不清楚，次级染色体不稳定发生时的表型和适合度结果也是未知的。在这里，我们报告了一个来自相对稳定的合成异源四倍体小麦的单个植株的特定谱系，其基因组为SLSLAA(S1和A亚基因组来自Ae。分别为Longissima和T.urartu)，表现出高度的跨代染色体不稳定性。染色体数目变异(NCV)和结构染色体变异(SCV)均广泛存在。虽然在不同的染色体上检测到NCV和SCV的频率都有很大的差异，但只有NCV的频率在两个亚基因组之间有显著差异。我们发现，NCVS和SCV的发生主要是由于受扰的减数分裂，允许形成多价体和单价体以及同源交换。因此，NCVS和SCV的组合影响了多个表型性状，特别是那些与生殖适合性相关的性状。
Newly formed plant allopolyploids usually have meiosis defect, resulting in chromosomal instability manifested as variation in chromosome number and/or structure. However, not all nascent allopolyploids are equally unstable. The wheat group (Aegilops/Triticum) contains 13 diploid species with distinct genome types. Many of these species can be artificially hybridized to produce viable but sterile inter-specific/intergeneric F1 hybrids, which can generate fertile synthetic allotetraploid wheats after whole genome doubling. Compared with synthetic allotetraploid wheats that contain genome combinations of AADD and S*S*DD (S* refers to related S genomes of a different species), those containing an S*S*AA genome are significantly more stable. However, robustness of the relative stability of S*S*AA genomes is unknown, nor are the phenotypic and fitness consequences during occurrences of secondary chromosomal instability. Here, we report a specific lineage originated from a single individual plant of a relatively stable synthetic allotetraploid wheat with genomes SlSlAA (Sl and A subgenomes were from Ae. longissima and T. urartu, respectively) that showed a high degree of transgenerational chromosomal instability. Both numerical chromosome variation (NCV) and structural chromosome variation (SCV) occurred widely. While substantial differences in frequencies of both NCV and SCV were detected across the different chromosomes, only NCV frequencies were significantly different between the two subgenomes. We found that NCVs and SCVs occurred primarily due to perturbed meiosis, allowing formation of multivalents and univalents as well as homoeologous exchanges. Thus, the combination of NCVs and SCVs affected multiple phenotypic traits, particularly those related to reproductive fitness.
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DOI：
10.1139/g72-004
发表时间：
1972-01-01
期刊：
CANADIAN JOURNAL OF GENETICS AND CYTOLOGY
影响因子：
--
作者：
DRISCOLL, CJ
通讯作者：
DRISCOLL, CJ
影响因子：
64.5
作者：
BEDBROOK, JR;JONES, J;FLAVELL, RB
通讯作者：
FLAVELL, RB
影响因子：
2.6
作者：
Endo, T. R.
通讯作者：
Endo, T. R.
DOI：
10.1038/newbio230022a0
发表时间：
1971-01-01
期刊：
NATURE-NEW BIOLOGY
影响因子：
--
作者：
MELLOSAMPAYO, T
通讯作者：
MELLOSAMPAYO, T
影响因子：
5.4
作者：
Han, FP;Liu, B;Liu, ZH
通讯作者：
Liu, ZH