Characterization of specific pancreatic polypeptide receptors on basolateral membranes of the canine small intestine.
Characterization of specific pancreatic polypeptide receptors on basolateral membranes of the canine small intestine.
复制标题
犬小肠基底外侧膜上特定胰多肽受体的表征。
DOI：
10.1073/pnas.85.13.4745
复制
发表时间：
1988
影响因子：
11.1
通讯作者：
Gingerich,RL
中科院分区：
文献类型：
--
作者：
Gilbert,WR;Frank,BH;Gavin3rd,JR;Gingerich,RL
我们在犬小肠粘膜层上发现胰多肽（PP）的特异性结合位点。本研究进行进一步表征纯化的肠膜上的这些结合位点（受体），并建立它们在刷状缘或肠上皮细胞基底外侧表面上的位置。通过山梨醇密度离心制备基底外侧和刷状缘膜。PP受体主要定位于血管表面，因此使用Tyr-27上标记的PP 125 I与基底外侧制剂的结合来评价受体特征。PP的结合是钙、时间、温度和pH依赖性的。标记PP的最大特异性结合发生在4 ℃下与5 mM钙在pH 6.8下孵育8小时后。Scatchard图的数据分析显示，高亲和力和低亲和力结合位点的相对亲和力分别为1.5 x 10（-9）M和2.6 x 10（-8）M，相应的结合容量分别为0.23 pmol/mg和0.84 pmol/mg蛋白质。这种受体是PP特异性的，因为肽YY和神经肽Y，肽的PP家族，交叉反应小于3%，从比较的一半最大位移的标签。结构不同的肽，胰岛素和胰高血糖素，没有竞争结合。特异性125 I标记的PP结合主要定位于基底外侧膜（9.8 +/- 0.8%），刷状缘膜几乎没有结合（0.8 +/- 0.2%）。因此，我们已经确定了高度特异性的受体PP，主要位于血管表面的小肠粘膜。这些数据表明，小肠粘膜衬里是PP的靶组织，PP参与小肠粘膜中燃料代谢和底物转运的激素调节。
We have identified specific binding sites for pancreatic polypeptide (PP) on the mucosal lining of canine small intestine. The present study was undertaken to further characterize these binding sites (receptors) on purified intestinal membranes and to establish their location on the brush border or basolateral surface of the intestinal enterocyte. Basolateral and brush border membranes were prepared by sorbitol density centrifugation. PP receptors were localized predominantly to the vascular surface, and thus binding of PP 125I-labeled on Tyr-27 to the basolateral preparation was used to evaluate receptor characteristics. Binding of PP was calcium, time, temperature, and pH dependent. Maximum specific binding of labeled PP occurred after an 8-hr incubation at 4 degrees C with 5 mM calcium at pH 6.8. Data analysis by Scatchard plot showed high- and low-affinity binding sites with relative affinities of 1.5 x 10(-9) M and 2.6 x 10(-8) M and with corresponding binding capacities of 0.23 pmol/mg and 0.84 pmol/mg of protein, respectively. This receptor was specific for PP since peptide YY and neuropeptide Y, peptides of the PP family, cross-reacted by less than 3%, as judged from comparisons of half-maximal displacement of label. Structurally dissimilar peptides, insulin and glucagon, did not compete for binding. Specific 125I-labeled PP binding was localized primarily to basolateral membranes (9.8 +/- 0.8%) with little binding by brush border membranes (0.8 +/- 0.2%). Thus, we have identified highly specific receptors for PP, located predominantly on the vascular surface of the small intestinal mucosa. These data suggest that the mucosal lining of the small intestine is a target tissue for PP and that PP participates in the hormonal regulation of fuel metabolism and substrate transport in the small intestinal mucosa.
人胰多肽及其C端六肽对人和狗胰腺分泌的影响。
影响因子：
1.9
作者：
S. Konturek;C. Meyers;N. Kwiecień;W. Obtułowicz;J. Tasler;J. Oleksy;B. Kopp;D. H. Coy;A. V. Schally
通讯作者：
A. V. Schally
肽 YY 与大鼠肠上皮质膜的相互作用：放射性碘标记肽的结合。
影响因子：
4.8
作者：
M. Laburthe;B. Chenut;C. Rouyer‐Fessard;K. Tatemoto;A. Couvineau;A. Servin;B. Amiranoff
通讯作者：
B. Amiranoff