The conserved oligomeric Golgi complex is involved in double-membrane vesicle formation during autophagy.
The conserved oligomeric Golgi complex is involved in double-membrane vesicle formation during autophagy.
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DOI：
10.1083/jcb.200904075
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发表时间：
2010-01-11
期刊：
影响因子：
--
通讯作者：
Klionsky DJ
中科院分区：
文献类型：
--
作者：
Yen WL;Shintani T;Nair U;Cao Y;Richardson BC;Li Z;Hughson FM;Baba M;Klionsky DJ
COG亚基定位于自噬体组装位点，在该位点它们与自噬蛋白相互作用，并且是双膜Cvt囊泡和自噬体形成所必需的。
巨自噬是真核细胞中用于长寿命蛋白质和细胞器周转的一种分解代谢途径。这一过程的形态学标志是形成包裹细胞质的双膜自噬体。自噬体的形成是巨自噬中最复杂的部分，它是一个动态事件，可能涉及囊泡融合以扩展最初包裹的膜——自噬泡；然而，对于这一过程，包括参与囊泡束缚和融合的分子成分，基本上一无所知。在这项研究中，我们提供的证据表明，保守的寡聚高尔基（COG）复合体的亚基是双膜细胞质到液泡靶向囊泡和自噬体形成所必需的。COG亚基定位于自噬泡组装位点，并与Atg（自噬相关）蛋白相互作用。此外，COG基因的突变导致Atg8和Atg9的错误定位，而Atg8和Atg9是参与自噬体形成的关键成分。
COG subunits localize to the phagophore assembly site where they interact with autophagy proteins and are required for double-membrane Cvt vesicle and autophagosome formation. Macroautophagy is a catabolic pathway used for the turnover of long-lived proteins and organelles in eukaryotic cells. The morphological hallmark of this process is the formation of double-membrane autophagosomes that sequester cytoplasm. Autophagosome formation is the most complex part of macroautophagy, and it is a dynamic event that likely involves vesicle fusion to expand the initial sequestering membrane, the phagophore; however, essentially nothing is known about this process including the molecular components involved in vesicle tethering and fusion. In this study, we provide evidence that the subunits of the conserved oligomeric Golgi (COG) complex are required for double-membrane cytoplasm to vacuole targeting vesicle and autophagosome formation. COG subunits localized to the phagophore assembly site and interacted with Atg (autophagy related) proteins. In addition, mutations in the COG genes resulted in the mislocalization of Atg8 and Atg9, which are critical components involved in autophagosome formation.
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影响因子：
7.8
作者：
Baba, M;Osumi, M;Scott, SV;Klionsky, DJ;Ohsumi, Y
通讯作者：
Ohsumi, Y
影响因子：
3.3
作者：
Cheong, H;Yorimitsu, T;Klionsky, DJ
通讯作者：
Klionsky, DJ
影响因子：
64.5
作者：
Albertini, M;Rehling, P;Kunau, WH
通讯作者：
Kunau, WH
影响因子：
3.3
作者：
Kim, DW;Sacher, M;Ferro-Novick, S
通讯作者：
Ferro-Novick, S
影响因子：
4.8
作者：
Bruinsma, P;Spelbrink, RG;Nothwehr, SF
通讯作者：
Nothwehr, SF