Comparison of two methods for assaying reducing sugars in the determination of carbohydrase activities.
Comparison of two methods for assaying reducing sugars in the determination of carbohydrase activities.
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DOI：
10.1155/2011/283658
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发表时间：
2011
影响因子：
1.8
通讯作者：
Sinitsyn AP
中科院分区：
文献类型：
--
作者：
Gusakov AV;Kondratyeva EG;Sinitsyn AP
Nelson-Somogyi(NS)法和3，5-二硝基水杨酸(3，5-DNSA)法被广泛用于测定碳水化合物对不同多糖的活性。以12种市售酶制剂为原料，比较了NS法和DNS法测定纤维素酶、β-葡聚糖酶、木聚糖酶和β-甘露聚糖酶的活力。当测定纤维素酶对CMC的活性时，DNS法给出的活力值通常比NS法高40-50%。在木聚糖酶、β-甘露聚糖酶和β-葡聚糖酶活性的分析中，dns法的高估要明显得多(观察到的活性差异是3-13倍)。讨论了在测定纤维素酶以外的碳水化合物酶活力时优先使用NS法的原因。
The Nelson-Somogyi (NS) and 3,5-dinitrosalicylic acid (DNS) assays for reducing sugars are widely used in measurements of carbohydrase activities against different polysaccharides. Using twelve commercial enzyme preparations, the comparison of the NS and DNS assays in determination of cellulase, β-glucanase, xylanase, and β-mannanase activities was carried out. When cellulase activities against CMC were measured, the DNS assay gave activity values, which were typically 40–50% higher than those obtained with the NS assay. In the analysis of the xylanase, β-mannanase, and β-glucanase activities, the overestimations by the DNS assay were much more pronounced (the observed differences in the activities were 3- to 13-fold). Reasons for preferential use of the NS assay for measuring activities of carbohydrases other than cellulases are discussed.
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DOI：
10.1016/j.bbagen.2004.07.001
发表时间：
2004-11-01
影响因子：
3
作者：
Grishutin, SG;Gusakov, AV;Sinitsyn, AP
通讯作者：
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影响因子：
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影响因子：
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作者：
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