Oral Delivery of Nisin in Resistant Starch Based Matrices Alters the Gut Microbiota in Mice.
Oral Delivery of Nisin in Resistant Starch Based Matrices Alters the Gut Microbiota in Mice.
复制标题
DOI：
10.3389/fmicb.2018.01186
复制
发表时间：
2018
影响因子：
5.2
通讯作者：
Rea MC
中科院分区：
文献类型：
--
作者：
Gough R;Cabrera Rubio R;O'Connor PM;Crispie F;Brodkorb A;Miao S;Hill C;Ross RP;Cotter PD;Nilaweera KN;Rea MC
人们越来越多地认识到胃肠道微生物区系在健康和疾病中的作用。摄入的抗菌蛋白和多肽有可能改变胃肠道微生物区系；特别是在防止消化的情况下。Nisin是一种用作食品防腐剂的抗菌肽。这项研究测试了Nisin在两种不同的淀粉基质中喂养小鼠时对小鼠微生物区系的影响的能力；一种是由生淀粉颗粒组成的淀粉团，另一种是由糊化和退化的淀粉组成的淀粉凝胶。考察了两种淀粉基质各自对微生物区系的影响。在16SRRNA组成测序之后，β多样性分析突出了四个饮食组之间小鼠微生物区系的显著差异(p=0.001，n=10)。两种乳链菌素饲料之间的差异主要归因于从淀粉基质中释放乳链菌素的差异，而载体之间的差异主要归因于它们所拥有的抗性淀粉的类型。事实上，在食用淀粉面团和淀粉凝胶饲料的小鼠中，几个属的相对丰度的差异，特别是阿克曼属，其相对丰度分别为0.5%和11.9%(p=0.0002，n=10)，表明抗性淀粉作为有益肠道微生物的调节剂的潜在价值。在粪便中检测到完整的Nisin和Nisin消化产物(特别是Nisin片段22-31)，该Nisin具有生物活性。然而，尽管在淀粉面团饮食中摄入Nisin的那组人的Nisin摄入量增加了三倍，但在淀粉凝胶饮食中摄入Nisin的那组人的粪便中检测到的Nisin是Nisin的两倍。此外，在淀粉凝胶饲料中饲喂Nisin的组，来自下胃肠道(Git)的3倍于对照组的相对丰度有显著差异(p<；0.001，n=10)，这表明淀粉凝胶对包裹在其中的Nisin具有一定程度的消化保护作用。
There is a growing recognition of the role the gastrointestinal microbiota plays in health and disease. Ingested antimicrobial proteins and peptides have the potential to alter the gastrointestinal microbiota; particularly if protected from digestion. Nisin is an antimicrobial peptide that is used as a food preservative. This study examined the ability of nisin to affect the murine microbiota when fed to mice in two different starch based matrices; a starch dough comprising raw starch granules and a starch gel comprising starch that was gelatinized and retrograded. The effects of the two starch matrices by themselves on the microbiota were also examined. Following 16S rRNA compositional sequencing, beta diversity analysis highlighted a significant difference (p = 0.001, n = 10) in the murine microbiota between the four diet groups. The differences between the two nisin containing diets were mainly attributable to differences in the nisin release from the starch matrices while the differences between the carriers were mainly attributable to the type of resistant starch they possessed. Indeed, the differences in the relative abundance of several genera in the mice consuming the starch dough and starch gel diets, in particular Akkermansia, the relative abundance of which was 0.5 and 11.9%, respectively (p = 0.0002, n = 10), points to the potential value of resistance starch as a modulator of beneficial gut microbes. Intact nisin and nisin digestion products (in particular nisin fragment 22–31) were detected in the feces and the nisin was biologically active. However, despite a three-fold greater consumption of nisin in the group fed the nisin in starch dough diet, twice as much nisin was detected in the feces of the group which consumed the nisin in starch gel diet. In addition, the relative abundance of three times as many genera from the lower gastrointestinal tract (GIT) were significantly different (p < 0.001, n = 10) to the control for the group fed the nisin in starch gel diet, implying that the starch gel afforded a degree of protection from digestion to the nisin entrapped within it.
登录
查看更多内容
通过饮食纤维对人肠道微生物群的调节发生在物种水平上。
影响因子：
5.4
作者：
Chung WS;Walker AW;Louis P;Parkhill J;Vermeiren J;Bosscher D;Duncan SH;Flint HJ
通讯作者：
Flint HJ
影响因子：
2.4
作者：
Corbett, Adrian;McGowin, Audrey;Sibbitt, Bethany
通讯作者：
Sibbitt, Bethany
DOI：
10.1111/j.1574-695x.1999.tb01262.x
发表时间：
1999-05-01
影响因子：
--
作者：
de Pablo, MA;Gaforio, JJ;López, GAD
通讯作者：
López, GAD
影响因子：
4.4
作者：
Bernbom, N;Licht, TR;Norrung, B
通讯作者：
Norrung, B
影响因子：
3.5
作者：
Chan, WC;Leyland, M;Roberts, GCK
通讯作者：
Roberts, GCK