Rapid Staining of Circulating Tumor Cells in Three-Dimensional Microwell Dialysis (3D-μDialysis) Chip.
Rapid Staining of Circulating Tumor Cells in Three-Dimensional Microwell Dialysis (3D-μDialysis) Chip.
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DOI：
10.1038/s41598-017-09829-3
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发表时间：
2017-09-12
影响因子：
4.6
通讯作者：
Jiang JK
中科院分区：
文献类型：
--
作者：
Cho W;Pradhan R;Chen HY;Weng YH;Chu HY;Tseng FG;Lin CP;Jiang JK
检测循环肿瘤细胞 (CTC) 的传统技术耗时较长，并且该技术中使用的离心力可能会导致细胞死亡。由于患者体内的 CTC 数量相当低，本研究旨在采用更温和的诊断程序，以免在样品制备过程中丢失太多 CTC。因此，本研究设计了三维微孔透析（3D-μDialysis）芯片，通过使用透析型流动过程而无需离心来执行温和的荧光去除过程。这使得我们研究中获得的 CTC 的手动处理最少，没有任何污染。此外，通过采用透析过程（通过一次动态去除水和废物）而不是仅静态扩散（通过扩散仅静态去除废物）来说明快速染色过程只需要传统技术一半的时间（35分钟而不是90分钟）。由于 3D-μ透析染色中的流速，我们技术的染色效率比传统染色有所提高。此外，染色过程已通过三名 TNM IV 期结肠癌患者的临床全血样本进行了验证。目前的技术可称为“微型快速染色和透析系统”。
The conventional techniques to detect circulating tumour cells (CTCs) are lengthy and the use of centrifugal forces in this technique may cause cell mortality. As the number of CTCs in patients is quite low, the present study aims towards a gentler diagnostic procedure so as not to lose too many CTCs during the sample preparation process. Hence, a Three-Dimensional Microwell dialysis (3D-μDialysis) chip was designed in this study to perform gentle fluorescence-removal process by using dialysis-type flow processes without centrifuging. This leads to a minimum manual handling of CTCs obtained in our study without any contamination. In addition, a rapid staining process which necessitates only about half the time of conventional techniques (35 minutes instead of 90 minutes) is being illustrated by the employment of dialysis process (by dynamically removing water and waste at once) instead of only static diffusion (by statically removing only waste by diffusion). Staining efficiency of our technique is improved over conventional staining because of the flow rate in 3D-μDialysis staining. Moreover, the staining process has been validated with clinical whole blood samples from three TNM stage IV colon cancer patients. The current technique may be termed as “miniature rapid staining and dialysing system”.
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影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
影响因子：
3.7
作者：
Xu, Lei;Mao, Xueying;Lu, Yong-Jie
通讯作者：
Lu, Yong-Jie
影响因子：
17.1
作者：
Ozkumur E;Shah AM;Ciciliano JC;Emmink BL;Miyamoto DT;Brachtel E;Yu M;Chen PI;Morgan B;Trautwein J;Kimura A;Sengupta S;Stott SL;Karabacak NM;Barber TA;Walsh JR;Smith K;Spuhler PS;Sullivan JP;Lee RJ;Ting DT;Luo X;Shaw AT;Bardia A;Sequist LV;Louis DN;Maheswaran S;Kapur R;Haber DA;Toner M
通讯作者：
Toner M
影响因子：
3.4
作者：
Balic, M;Dandachi, N;Bauernhofer, T
通讯作者：
Bauernhofer, T
影响因子：
12.6
作者：
Hyun, Kyung-A;Lee, Tae Yoon;Jung, Hyo-Il
通讯作者：
Jung, Hyo-Il