ParAB Partition Dynamics in Firmicutes: Nucleoid Bound ParA Captures and Tethers ParB-Plasmid Complexes.
ParAB Partition Dynamics in Firmicutes: Nucleoid Bound ParA Captures and Tethers ParB-Plasmid Complexes.
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DOI：
10.1371/journal.pone.0131943
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Alonso JC
中科院分区：
文献类型：
--
作者：
Lioy VS;Volante A;Soberón NE;Lurz R;Ayora S;Alonso JC
在厚壁菌门中，小的同源二聚体ParA样（δ2）和ParB样（ω2）蛋白与顺式作用质粒携带的parS和宿主染色体协同作用，在不断增长的细菌群体中确保稳定的质粒遗传。这项研究表明，（ω：YFP）2结合parS促进质粒在胞质溶胶中的聚类。（δ：GFP）2需要ATP结合而不是水解以定位到细胞的类核上作为荧光云。结合至类核的（δ：CFP）2或δ2与（ω：YFP）2焦点的相互作用促进质粒从非常广泛的分布捕获至类核和质粒配对。parS结合的ω2促进（δ：GFP）2的再分布，导致（δ：GFP）2从类核中动态释放，这一过程受到ATP水解和蛋白质-蛋白质相互作用的促进。（δ D 60 A：GFP）2，结合但不能水解ATP，也在类核上形成不稳定的复合物。在ω2的存在下，（δ D 60 A：GFP）2在类核上积累病灶或补丁结构。我们认为（δ：GFP）2与不同的类核区域和ω2-parS结合可能产生（δ：GFP）2梯度，可以指导质粒移动。重复的配对和解配对循环可以将质粒等距离地拴系在类核上，以确保通过与如从体外重构系统提出的扩散棘轮机制相容的机制进行忠实的质粒分离。
In Firmicutes, small homodimeric ParA-like (δ2) and ParB-like (ω2) proteins, in concert with cis-acting plasmid-borne parS and the host chromosome, secure stable plasmid inheritance in a growing bacterial population. This study shows that (ω:YFP)2 binding to parS facilitates plasmid clustering in the cytosol. (δ:GFP)2 requires ATP binding but not hydrolysis to localize onto the cell’s nucleoid as a fluorescent cloud. The interaction of (δ:CFP)2 or δ2 bound to the nucleoid with (ω:YFP)2 foci facilitates plasmid capture, from a very broad distribution, towards the nucleoid and plasmid pairing. parS-bound ω2 promotes redistribution of (δ:GFP)2, leading to the dynamic release of (δ:GFP)2 from the nucleoid, in a process favored by ATP hydrolysis and protein-protein interaction. (δD60A:GFP)2, which binds but cannot hydrolyze ATP, also forms unstable complexes on the nucleoid. In the presence of ω2, (δD60A:GFP)2 accumulates foci or patched structures on the nucleoid. We propose that (δ:GFP)2 binding to different nucleoid regions and to ω2-parS might generate (δ:GFP)2 gradients that could direct plasmid movement. The iterative pairing and unpairing cycles may tether plasmids equidistantly on the nucleoid to ensure faithful plasmid segregation by a mechanism compatible with the diffusion-ratchet mechanism as proposed from in vitro reconstituted systems.
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