Frequent deletion in chromosome 4 and duplication of chromosome 15 in liver epithelial cells derived from long‐term culture of C3H mouse hepatocytes
Frequent deletion in chromosome 4 and duplication of chromosome 15 in liver epithelial cells derived from long‐term culture of C3H mouse hepatocytes
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C3H小鼠肝细胞长期培养的肝上皮细胞中4号染色体频繁缺失和15号染色体重复
DOI：
10.1002/ijc.2910590120
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发表时间：
1994
影响因子：
6.4
通讯作者：
H. Tateno
中科院分区：
文献类型：
--
作者：
H. Nishimori;K. Ogawa;H. Tateno
从小鼠肝脏分离的肝细胞的延长培养导致肝上皮细胞集落的自发发育，其可以在体外无限增殖。我们从C3 H/HeJ小鼠（C3 H）和C3 H/HeJ × C57 BL/6 J F1小鼠（C3 B6 F1）分别建立了5个和22个这样的细胞系，以研究这些细胞的发育是否与任何特定的核型改变有关。这些细胞系保留了肝细胞的一些特性，并显示出胆管细胞特征，主要由近二倍体和/或亚四倍体细胞组成。C3 H细胞系的核型分析表明，大多数细胞具有涉及C7部分的4号染色体缺失或缺失，而4号染色体的至少1个（对于近二倍体细胞）或2个（对于亚四倍体细胞）拷贝通常是完整的。此外，在这些细胞系中经常观察到额外的15号染色体的获得。对22个C3 B6 F1株系的微卫星DNA多态性标记分析表明，大多数株系在第4染色体上的3个多态位点中至少有1个位点出现杂合性丢失（洛），而在第7和第11染色体上的2个位点未出现杂合性丢失。因此，小鼠4号和15号染色体可能含有与此类肝上皮细胞体外无限生长能力相关的基因[4号染色体上的基因座被指定为肝细胞永生化（LCI）基因座]。
Prolonged culture of hepatocytes isolated from mouse liver results in the spontaneous development of colonies of liver epithelial cells that can proliferate indefinitely in vitro. We established 5 such cell lines from C3H/HeJ mice (C3H) and 22 cell lines from C3H/HeJ × C57BL/6J F1 mice (C3B6F1) to investigate whether any specific karyotype alterations may be associated with the development of such cells. These lines retained some properties of hepatocytes as well as showing bile‐duct‐cell characteristics, and comprised mainly near‐diploid and/or hypotetraploid cells. Karyotypic analysis of the C3H cell lines indicated that most cells have loss of chromosome 4 or deletion involving the C7 portion, while at least I (for near‐diploid cells) or 2 (for hypotetraploid cells) copies of chromosome 4 were usually intact. In addition, gain of an extra chromosome 15 was frequently observed in these cell lines. Analysis of the microsatellite DNA polymorphic markers in 22 C3B6F1 lines revealed that a majority of them showed loss of heterozygosity (LOH) for, at least, 1 of 3 polymorphic loci on chromosome 4, but not for 2 loci on chromosomes 7 and 11. Mouse chromosomes 4 and 15, therefore, may contain genes related to the ability of such liver epithelial cells to grow indefinitely in vitro [The locus on chromosome 4 was designated as liver‐cell immortalization (LCI) locus].
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1 号或 14 号染色体杂合性缺失定义了晚期神经母细胞瘤的亚型。
影响因子：
11.2
作者：
Fong,CT;White,PS;Peterson,K;Sapienza,C;Cavenee,WK;Kern,SE;Vogelstein,B;Cantor,AB;Look,AT;Brodeur,GM
通讯作者：
Brodeur,GM
鉴定正常人类基因组中对于抑制仓鼠细胞转化至关重要的单个染色体。
DOI：
10.1073/pnas.82.2.570
发表时间：
1985
影响因子：
11.1
作者：
Stoler,A;Bouck,N
通讯作者：
Bouck,N
DOI：
10.1073/pnas.85.14.5259
发表时间：
1988-07-01
影响因子：
11.1
作者：
DIAZ, MO;ZIEMIN, S;ROWLEY, JD
通讯作者：
ROWLEY, JD
DOI：
10.1073/pnas.89.21.10557
发表时间：
1992-11-01
影响因子：
11.1
作者：
FOUNTAIN, JW;KARAYIORGOU, M;DRACOPOLI, NC
通讯作者：
DRACOPOLI, NC
适合进行种内杂交分型的小鼠遗传图谱。
影响因子：
3.3
作者：
Dietrich,W;Katz,H;Lincoln,SE;Shin,HS;Friedman,J;Dracopoli,NC;Lander,ES
通讯作者：
Lander,ES