Altered activation state of circulating neutrophils in patients with neovascular age-related macular degeneration.
Altered activation state of circulating neutrophils in patients with neovascular age-related macular degeneration.
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DOI：
10.1186/s12979-017-0100-9
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Sørensen TL
中科院分区：
文献类型：
--
作者：
Krogh Nielsen M;Hector SM;Allen K;Subhi Y;Sørensen TL
中性粒细胞功能障碍在以炎症和血管生成为特征的疾病的发展中起着关键作用。在这里，我们研究了新生血管性年龄相关性黄斑变性（AMD）患者中反映炎症激活、粘附和消退的中性粒细胞标志物的全身表达。这是一项针对新生血管性 AMD 患者和年龄匹配的健康对照个体的前瞻性病例对照研究。患者是从门诊项目中招募的，对照个体是从患者的亲属中招募的。当前吸烟者和患有活动性免疫疾病或正在进行的癌症的个体不包括在内，因为已知这些因素会影响中性粒细胞功能。对新鲜抽取的静脉血进行处理，用于中性粒细胞标记物的流式细胞术分析。我们确定阳性细胞的百分比并使用荧光强度测量比较表达水平。我们发现新生血管性 AMD 患者 (n = 29) 和对照组 (n = 28) 之间标记物表达存在条件差异：广泛观察时没有发现差异，但当关注非吸烟者时出现了一些差异。在此，新生血管性 AMD 患者的分化活性标志物簇 (CD) 66b 的表达增加（P = 0.003；Mann-Whitney U 检验），粘附标志物 CD162 的表达减少（P = 0.044；Mann-Whitney U 检验），炎症标志物 C-X-C 趋化因子受体 2 的分辨率表达降低（P = 0.044；Mann-Whitney U 检验）。我们提出的新证据表明，对吸烟敏感的循环中性粒细胞的活性在新生血管性 AMD 患者中可能有所不同。
Neutrophil dysfunction plays a key role in the development of diseases characterized by inflammation and angiogenesis. Here, we studied the systemic expression of neutrophil markers reflecting activation, adhesion, and resolution of inflammation in patients with neovascular age-related macular degeneration (AMD). This was a prospective case-control study of patients with neovascular AMD and age-matched healthy control individuals. Patients were recruited from an outpatient program, and control individuals were recruited amongst patients’ relatives. Current smokers and individuals with either active immune-disease or ongoing cancer were not included, as these factors are known to affect neutrophil function. Fresh-drawn venous blood was processed for flow cytometric analysis of neutrophil markers. We determined percentages of positive cells and compared expression levels using fluorescence intensity measures. We found conditional differences on marker expression between patients with neovascular AMD (n = 29) and controls (n = 28): no differences were found when looking broadly, but several differences emerged when focusing on non-smokers. Here, patients with neovascular AMD had increased expression of the activity marker cluster of differentiation (CD) 66b (P = 0.003; Mann-Whitney U test), decreased expression of adhesion marker CD162 (P = 0.044; Mann-Whitney U test), and lower expression of the resolution of inflammation marker C-X-C chemokine receptor 2 (P = 0.044; Mann-Whitney U test). We present novel evidence suggesting that the activity of circulating neutrophils, sensitive to smoking, may differ in patients with neovascular AMD.
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