Blood leukocyte Alu and LINE-1 methylation and gastric cancer risk in the Shanghai Women's Health Study.
Blood leukocyte Alu and LINE-1 methylation and gastric cancer risk in the Shanghai Women's Health Study.
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DOI：
10.1038/bjc.2011.562
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发表时间：
2012-01-31
影响因子：
8.8
通讯作者：
Chow, W-H
中科院分区：
文献类型：
--
作者：
Gao, Y.;Baccarelli, A.;Shu, X. O.;Ji, B-T;Yu, K.;Tarantini, L.;Yang, G.;Li, H-L;Hou, L.;Rothman, N.;Zheng, W.;Gao, Y-T;Chow, W-H
最近的数据表明血液白细胞 DNA 甲基化与癌症风险之间存在联系。然而，目前还没有关于胃癌的前瞻性研究的 DNA 甲基化报告。我们在一项前瞻性上海女性健康研究队列中的病例对照研究中探讨了诊断前血液白细胞 DNA 甲基化与胃癌风险之间的关联。该研究包括胃癌病例 (n=192) 和匹配对照 (n=384)。使用亚硫酸氢盐焦磷酸测序评估 Alu 和长散布核苷酸元件 (LINE)-1 的甲基化。比值比 (OR) 和 95% 置信区间 (CI) 通过对潜在混杂因素进行逻辑回归调整来计算。 Alu 甲基化与胃癌风险呈负相关，主要是在采血一年或多年后诊断的病例中。排除随访第一年诊断的病例后，Alu甲基化第三、第二和第一四分位数与最高四分位数相比的OR分别为2.43（1.43-4.13）、1.47（0.85-2.57）和2.22（1.28-3.84）。这种关联似乎会因饮食摄入量（尤其是异黄酮）而改变。相比之下，LINE-1 甲基化水平与胃癌风险无关。这项前瞻性研究的证据与血液白细胞 DNA 低甲基化可能与癌症风险（包括胃癌风险）相关的假设一致。
Recent data suggest a link between blood leukocyte DNA methylation, and cancer risk. However, reports on DNA methylation from a prospective study are unavailable for gastric cancer. We explored the association between methylation in pre-diagnostic blood leukocyte DNA and gastric cancer risk in a case–control study nested in the prospective Shanghai Women's Health Study cohort. Incident gastric cancer cases (n=192) and matched controls (n=384) were included in the study. Methylation of Alu and long interspersed nucleotide elements (LINE)-1 were evaluated using bisulphite pyrosequencing. Odds ratios (ORs) and 95% confidence intervals (CI) were calculated from logistic regression adjusting for potential confounders. Alu methylation was inversely associated with gastric cancer risk, mainly among cases diagnosed one or more years after blood collection. After excluding cases diagnosed during the first year of follow-up, the ORs for the third, second, and first quartiles of Alu methylation compared with the highest quartile were 2.43 (1.43–4.13), 1.47(0.85–2.57), and 2.22 (1.28–3.84), respectively. This association appeared to be modified by dietary intake, particularly isoflavone. In contrast, LINE-1 methylation levels were not associated with gastric cancer risk. Evidence from this prospective study is consistent with the hypothesis that DNA hypomethylation in blood leukocytes may be related to cancer risk, including risk of gastric cancer.
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影响因子：
4.5
作者：
Christensen BC;Houseman EA;Marsit CJ;Zheng S;Wrensch MR;Wiemels JL;Nelson HH;Karagas MR;Padbury JF;Bueno R;Sugarbaker DJ;Yeh RF;Wiencke JK;Kelsey KT
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作者：
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Linnebank, Michael
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作者：
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通讯作者：
Christiani DC
影响因子：
64.5
作者：
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通讯作者：
Moran, JV
影响因子：
11.2
作者：
Bollati, Valentina;Baccarelli, Andrea;Yang, Allen S.
通讯作者：
Yang, Allen S.