Testosterone insulin-like effects: an in vitro study on the short-term metabolic effects of testosterone in human skeletal muscle cells.
Testosterone insulin-like effects: an in vitro study on the short-term metabolic effects of testosterone in human skeletal muscle cells.
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DOI：
10.1007/s40618-017-0686-y
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发表时间：
2017-10
影响因子：
5.4
通讯作者：
Di Luigi L
中科院分区：
文献类型：
--
作者：
Antinozzi C;Marampon F;Corinaldesi C;Vicini E;Sgrò P;Vannelli GB;Lenzi A;Crescioli C;Di Luigi L
睾酮通过促进不同的代谢途径有助于骨骼肌的短期稳态，骨骼肌是最大的胰岛素敏感组织，也是胰岛素刺激葡萄糖利用的主要部位。尽管有证据表明睾酮和葡萄糖代谢之间存在密切关系，但睾酮介导的骨骼肌胰岛素样作用的分子机制仍然未知。在这里，我们使用未分化增殖或分化的人类胎儿骨骼肌细胞（Hfsmc）来研究睾酮对胰岛素介导的生物分子代谢机制的短期影响。分析睾酮治疗后不同时间点的 GLUT4 细胞表达、定位以及 AKT、ERK、mTOR 和 GSK3β 胰岛素相关通路的磷酸化/激活。独立于细胞分化状态，具有胰岛素样作用的睾酮诱导Glut4-mRNA表达、GLUT4蛋白易位至细胞质膜，但未观察到对GLUT4蛋白表达水平的影响。此外，睾酮治疗调节胰岛素依赖性信号转导途径，诱导 AKT、ERK 和 mTOR 快速且持续的激活，以及 GSK3β 的短暂抑制。 T 相关效应被证明是雄激素受体依赖性的。总之，我们的数据表明，睾酮通过非基因组途径的激活，通过诱导胰岛素相关效应参与骨骼肌葡萄糖代谢。
Testosterone by promoting different metabolic pathways contributes to short-term homeostasis of skeletal muscle, the largest insulin-sensitive tissue and the primary site for insulin-stimulated glucose utilization. Despite evidences indicate a close relationship between testosterone and glucose metabolism, the molecular mechanisms responsible for a possible testosterone-mediated insulin-like effects on skeletal muscle are still unknown. Here we used undifferentiated proliferating or differentiated human fetal skeletal muscle cells (Hfsmc) to investigate the short-term effects of testosterone on the insulin-mediated biomolecular metabolic machinery. GLUT4 cell expression, localization and the phosphorylation/activation of AKT, ERK, mTOR and GSK3β insulin-related pathways at different time points after treatment with testosterone were analyzed. Independently from cells differentiation status, testosterone, with an insulin-like effect, induced Glut4-mRNA expression, GLUT4 protein translocation to the cytoplasmic membrane, while no effect was observed on GLUT4 protein expression levels. Furthermore, testosterone treatment modulated the insulin-dependent signal transduction pathways inducing a rapid and persistent activation of AKT, ERK and mTOR, and a transient inhibition of GSK3β. T-related effects were shown to be androgen receptor dependent. All together our data indicate that testosterone through the activation of non-genomic pathways, participates in skeletal muscle glucose metabolism by inducing insulin-related effects.
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影响因子：
2.5
作者：
Di Luigi, L.;Baldari, C.;Guidetti, L.
通讯作者：
Guidetti, L.
影响因子：
5.8
作者：
Dhindsa, S;Prabhakar, S;Dandona, P
通讯作者：
Dandona, P
DOI：
10.1073/pnas.90.2.512
发表时间：
1993-01-15
影响因子：
11.1
作者：
FLORESRIVEROS, JR;MCLENITHAN, JC;LANE, MD
通讯作者：
LANE, MD
影响因子：
5.4
作者：
Crescioli, C.;Sturli, N.;Di Luigi, L.
通讯作者：
Di Luigi, L.
影响因子：
2.5
作者：
Felici, Francesco;Bazzucchi, Ilenia;Di Luigi, Luigi
通讯作者：
Di Luigi, Luigi