The miRNAome of the postpartum dairy cow liver in negative energy balance.
The miRNAome of the postpartum dairy cow liver in negative energy balance.
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DOI：
10.1186/1471-2164-15-279
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发表时间：
2014-04-12
期刊：
影响因子：
4.4
通讯作者：
Morris D
中科院分区：
文献类型：
--
作者：
Fatima A;Lynn DJ;O'Boyle P;Seoighe C;Morris D
负能量平衡（NEB）是高产奶牛在产后最初几周内发生的一种代谢状态改变，此时哺乳和维持的能量需求超过了饮食摄入的能量供应。NEB反过来又会导致代谢紊乱和生育能力下降。在产后奶牛的NEB研究中，已经报道了700多个肝脏基因表达的改变。已知mirna （microRNA）在转录后介导许多基因表达的改变。为了研究产后奶牛轻度NEB （MNEB）和重度NEB （SNEB）肝脏miRNA含量，包括其总体丰度和差异表达，我们进行了短读RNA测序。为了鉴定奶牛SNEB中差异表达肝脏基因中差异表达mirna的推定靶标，采用了计算靶标鉴定。我们的研究结果表明，奶牛肝脏以每百万10个reads的较低阈值表达53个mirna。其中，10个miRNA占miRNAome （miRNA含量）的95%以上。在高表达的mirna中，miR-122占75%，其次是miR-192和miR-3596。13个let-7 miRNA家族成员中有5个也是高表达的miRNA。在SNEB中下调的miR-143，被发现有4个推测的与SNEB相关的上调基因靶点，包括LRP2（低密度脂蛋白受体相关蛋白2），参与脂质代谢，在SNEB中上调。这是第一个中等产量奶牛产后早期肝脏miRNA-seq分析研究。组织特异性miR-122和肝脏富集miR-192是产后奶牛肝脏中含量最高的两种mirna。miR-143在SNEB中显著下调，而在SNEB中上调的miRNA-143的推测靶点包括一个参与脂质代谢的基因。
Negative energy balance (NEB) is an altered metabolic state in high yielding cows that occurs during the first few weeks postpartum when energy demands for lactation and maintenance exceed the energy supply from dietary intake. NEB can, in turn, lead to metabolic disorders and to reduced fertility. Alterations in the expression of more than 700 hepatic genes have previously been reported in a study of NEB in postpartum dairy cows. miRNAs (microRNA) are known to mediate many alterations in gene expression post transcriptionally. To study the hepatic miRNA content of postpartum dairy cows, including their overall abundance and differential expression, in mild NEB (MNEB) and severe NEB (SNEB), short read RNA sequencing was carried out. To identify putative targets of differentially expressed miRNAs among differentially expressed hepatic genes reported previously in dairy cows in SNEB computational target identification was employed. Our results indicate that the dairy cow liver expresses 53 miRNAs at a lower threshold of 10 reads per million. Of these, 10 miRNAs accounted for greater than 95% of the miRNAome (miRNA content). Of the highly expressed miRNAs, miR-122 constitutes 75% followed by miR-192 and miR-3596. Five out of thirteen let-7 miRNA family members are also among the highly expressed miRNAs. miR-143, down-regulated in SNEB, was found to have 4 putative up-regulated gene targets associated with SNEB including LRP2 (low density lipoprotein receptor-related protein 2), involved in lipid metabolism and up-regulated in SNEB. This is the first liver miRNA-seq profiling study of moderate yielding dairy cows in the early postpartum period. Tissue specific miR-122 and liver enriched miR-192 are two of the most abundant miRNAs in the postpartum dairy cow liver. miR-143 is significantly down-regulated in SNEB and putative targets of miRNA-143 which are up-regulated in SNEB, include a gene involved in lipid metabolism.
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DOI：
10.1016/j.bbagrm.2012.06.001
发表时间：
2012-11
影响因子：
4.7
作者：
Hou, Weihong;Tian, Qing;Steuerwald, Nury M.;Schrum, Laura W.;Bonkovsky, Herbert L.
通讯作者：
Bonkovsky, Herbert L.
影响因子：
4.2
作者：
Becker, Christiane;Riedmaier, Irmgard;Meyer, Heinrich H. D.
通讯作者：
Meyer, Heinrich H. D.
影响因子：
4.6
作者：
Coutinho, Luiz L.;Matukumalli, Lakshmi K.;Smith, Timothy P. L.
通讯作者：
Smith, Timothy P. L.
影响因子：
14.9
作者：
Betel D;Wilson M;Gabow A;Marks DS;Sander C
通讯作者：
Sander C
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y