Ca(2+) signaling in arterioles and small arteries of conscious, restrained, optical biosensor mice.
Ca(2+) signaling in arterioles and small arteries of conscious, restrained, optical biosensor mice.
复制标题
DOI：
10.3389/fphys.2014.00387
复制
发表时间：
2014
影响因子：
4
通讯作者：
Wier WG
中科院分区：
文献类型：
--
作者：
Fairfax ST;Mauban JR;Hao S;Rizzo MA;Zhang J;Wier WG
双光子荧光显微镜和清醒的，限制的光学生物传感器小鼠被用来研究平滑肌Ca 2+信号在耳小动脉。清醒的小鼠用于保持正常的平均动脉血压（MAP）和交感神经活动（SNA）。ExMLCK小鼠，表达基因编码的平滑肌特异性FRET为基础的Ca 2+指标，配备了血压遥测和固定成像。清醒束缚小鼠MAP为101 ± 4 mmHg，与自由移动的状态（107 ± 3 mmHg）相似。在大多数小动脉（71%）中观察到振荡性血管运动或不规则收缩，在0.25 s-1处观察到最大振荡频率。在平均直径约为35 μm的典型小动脉中，5-6 μm量级的振荡性血管运动伴随着约0.1 - 0.5 μM的几乎均匀的[Ca 2 +]振荡，最大[Ca 2 +]出现在直径快速减小之前。也观察到非常快速，空间均匀的“Ca 2+闪光”，但不是异步传播的Ca 2+波。与此相反，血管运动和动态Ca 2+信号很少观察到在耳小动脉麻醉exMLCK生物传感器小鼠。六甲铵（30 μg/g BW，i. p.）引起MAP下降至74 ± 4 mmHg，小动脉血管舒张，血管舒缩和同步Ca 2+瞬变消失。总结：在清醒、受限小鼠的高分辨率Ca 2+成像期间，MAP和心率（HR）正常。SNA通过动脉壁内空间均匀的Ca 2+信号诱导连续的血管运动和不规则的血管收缩。基于FRET的生物传感器小鼠和双光子成像首次测量了清醒动物小动脉血管平滑肌细胞中的[Ca 2 +]。
Two-photon fluorescence microscopy and conscious, restrained optical biosensor mice were used to study smooth muscle Ca2+ signaling in ear arterioles. Conscious mice were used in order to preserve normal mean arterial blood pressure (MAP) and sympathetic nerve activity (SNA). ExMLCK mice, which express a genetically-encoded smooth muscle-specific FRET-based Ca2+ indicator, were equipped with blood pressure telemetry and immobilized for imaging. MAP was 101 ± 4 mmHg in conscious restrained mice, similar to the freely mobile state (107 ± 3 mmHg). Oscillatory vasomotion or irregular contractions were observed in most arterioles (71%), with the greatest oscillatory frequency observed at 0.25 s−1. In a typical arteriole with an average diameter of ~35 μm, oscillatory vasomotion of a 5–6 μm magnitude was accompanied by nearly uniform [Ca2+] oscillations from ~0.1 to 0.5 μM, with maximum [Ca2+] occurring immediately before the rapid decrease in diameter. Very rapid, spatially uniform “Ca2+ flashes” were also observed but not asynchronous propagating Ca2+ waves. In contrast, vasomotion and dynamic Ca2+ signals were rarely observed in ear arterioles of anesthetized exMLCK biosensor mice. Hexamethonium (30 μg/g BW, i.p.) caused a fall in MAP to 74 ± 4 mmHg, arteriolar vasodilation, and abolition of vasomotion and synchronous Ca2+ transients. Summary: MAP and heart rate (HR) were normal during high-resolution Ca2+ imaging of conscious, restrained mice. SNA induced continuous vasomotion and irregular vasoconstrictions via spatially uniform Ca2+ signaling within the arterial wall. FRET-based biosensor mice and two-photon imaging provided the first measurements of [Ca2+] in vascular smooth muscle cells in arterioles of conscious animals.
登录
查看更多内容
影响因子：
56.9
作者：
NELSON, MT;CHENG, H;LEDERER, WJ
通讯作者：
LEDERER, WJ
DOI：
10.1152/ajpheart.2001.280.5.h2399
发表时间：
2001-05-01
影响因子：
4.8
作者：
Mauban, JRH;Lamont, C;Wier, WG
通讯作者：
Wier, WG
影响因子：
2.1
作者：
Ullman, J;Härgestam, R;Rundgren, M
通讯作者：
Rundgren, M
DOI：
10.1073/pnas.1100428108
发表时间：
2011-05-17
影响因子：
11.1
作者：
Drew, Patrick J.;Shih, Andy Y.;Kleinfeld, David
通讯作者：
Kleinfeld, David
影响因子：
5.5
作者：
Raina, H.;Zacharia, J.;Wier, W. G.
通讯作者：
Wier, W. G.