BSH-TRAP: Bile salt hydrolase tagging and retrieval with activity-based probes.
BSH-TRAP: Bile salt hydrolase tagging and retrieval with activity-based probes.
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DOI：
10.1016/bs.mie.2021.12.002
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发表时间：
2022
影响因子：
--
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中科院分区：
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胆汁酸是参与消化和调节包括新陈代谢和炎症在内的许多宿主生理过程的重要分子。初级胆汁酸是由肝脏中的胆固醇生物合成的，在分泌到肠道之前，它们在肝脏与甘氨酸和牛磺酸结合。这些分子中的一小部分保留在肠道中，在那里它们被一种微生物酶胆盐水解酶(BSH)修饰，胆盐水解酶可以解除甘氨酸和牛磺酸基团的共轭。这种去结合作用先于肠道中所有后续的生物转化，包括区域选择性脱羟化和异构化反应，以产生大量次生胆汁酸。因此，BSH被认为是次级胆汁酸代谢的守门酶，因此，它控制着宿主体内的总体胆汁酸组成。尽管BSH在胆汁酸代谢中起着关键作用，但在复杂的生物混合物中，很少有工具来探索它的活性。在本章中，我们描述了一种称为BSH-TRAP(基于活性的探针标记和检索胆盐水解酶)的化学蛋白质组学方法，该方法能够可视化和鉴定细菌中的BSH活性。在这里，我们描述了BSH陷阱在培养的细菌菌株和来自小鼠的肠道微生物中的应用。我们设想BSH-TRAP可以用于描述BSH活性的变化，并在复杂的生物样本中鉴定新的BSH酶，例如肠道微生物组。
Bile acids are important molecules that participate in digestion and regulate many host physiological processes, including metabolism and inflammation. Primary bile acids are biosynthesized from cholesterol in the liver, where they are conjugated to glycine and taurine before secretion into the intestines. A small fraction of these molecules remain in the gut, where they are modified by a microbial enzyme, bile salt hydrolase (BSH), which deconjugates the glycine and taurine groups. This deconjugation precedes all subsequent biotransformation in the intestines, including regioselective dehydroxylation and epimerization reactions, to produce numerous secondary bile acids. Thus, BSH is considered the gatekeeper enzyme of secondary bile acid metabolism, and, as a result, it controls the overall bile acid composition in the host. Despite the critical role that BSH plays in bile acid metabolism, there exist few tools to probe its activity in complex biological mixtures. In this chapter, we describe a chemoproteomic approach termed BSH-TRAP (bile salt hydrolase tagging and retrieval with activity-based probes) that enables visualization and identification of BSH activity in bacteria. Here, we describe application of BSH-TRAP to cultured bacterial strains and the gut microbes derived from mice. We envision that BSH-TRAP could be used to profile changes in BSH activity and identify novel BSH enzymes in complex biological samples, such as the gut microbiome.
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胆汁盐水解酶活性的无创成像和定量：从细菌到人。
影响因子：
13.6
作者：
Khodakivskyi PV;Lauber CL;Yevtodiyenko A;Bazhin AA;Bruce S;Ringel-Kulka T;Ringel Y;Bétrisey B;Torres J;Hu J;Chou CJ;Goun EA
通讯作者：
Goun EA
影响因子：
64.8
作者：
Devkota, Suzanne;Wang, Yunwei;Musch, Mark W.;Leone, Vanessa;Fehlner-Peach, Hannah;Nadimpalli, Anuradha;Antonopoulos, Dionysios A.;Jabri, Bana;Chang, Eugene B.
通讯作者：
Chang, Eugene B.
影响因子：
30.3
作者：
Sinha SR;Haileselassie Y;Nguyen LP;Tropini C;Wang M;Becker LS;Sim D;Jarr K;Spear ET;Singh G;Namkoong H;Bittinger K;Fischbach MA;Sonnenburg JL;Habtezion A
通讯作者：
Habtezion A
影响因子：
64.8
作者：
Song, Xinyang;Sun, Ximei;Kasper, Dennis L.
通讯作者：
Kasper, Dennis L.
影响因子：
64.5
作者：
Fischbach MA
通讯作者：
Fischbach MA