Changes in cytosolic ionized calcium induced by activators of secretion in GH3 cells.
Changes in cytosolic ionized calcium induced by activators of secretion in GH3 cells.
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GH3 细胞中分泌激活剂诱导的胞质离子钙的变化。
DOI：
10.1152/ajpendo.1984.246.2.e198
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Borle,AB
中科院分区：
文献类型：
--
作者：
Snowdowne,KW;Borle,AB
在低渗休克处理下，加入aequorin的GH3细胞中测定胞质离子钙（Cai）。给药10(-7)M促甲状腺激素释放激素（TRH）后，蔡由0.4 μ M上升到1.3 μ M；高钾浓度（60 mM）引起去极化后，蔡由0.3 μ M上升到1.1 μ M。反复的TRH刺激降低了Cai反应的强度。这种强度与刺激之间的间隔直接相关。在低钙培养基（0.13 mM Ca）中孵育0.5 h没有改变Cai对TRH的反应，但在无Ca培养基中孵育降低了反应。这些结果表明，TRH从内源性钙库中动员钙，钙可以被耗尽和补充。K去极化诱导的Cai升高较TRH慢（时间常数，TRH为t = 9 s， K去极化为t = 111 s）。即使在K去极化使Cai升高时，TRH仍可进一步升高，这表明这两种刺激从两个不同的池中动员钙，并通过不同的机制。
Cytosolic ionized calcium (Cai) was measured in GH3 cells with aequorin incorporated by hypoosmotic shock treatment. Cai rose from 0.4 to 1.3 microM after the administration of 10(-7) M thyrotropin-releasing hormone (TRH) and from 0.3 to 1.1 microM after depolarization caused by high-K concentration (60 mM). Repeated TRH stimulations decreased the magnitude of the Cai response. This magnitude was directly related to the interval between stimuli. Incubations in low-calcium media (0.13 mM Ca) for 0.5 h did not alter the Cai response to TRH, but incubations in Ca-free media reduced the response. These results suggest that TRH mobilized calcium from an endogenous pool of Ca that can be depleted and replenished. The rise in Cai induced by K depolarization was slower than that evoked by TRH (time constant, t = 9 s for TRH and t = 111 s for K depolarization). Even when Cai was elevated by K depolarization, it could still be further increased by TRH, suggesting that these two stimuli mobilize calcium from two different pools and by different mechanisms.
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