Asiatic acid ameliorates tubulointerstitial fibrosis in mice with ureteral obstruction.
Asiatic acid ameliorates tubulointerstitial fibrosis in mice with ureteral obstruction.
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亚洲酸可以改善输尿管阻塞的小鼠中的肾小管间隙纤维化。
DOI：
10.3892/etm.2013.1197
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发表时间：
2013-09
影响因子：
2.7
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Xu C;Wang W;Xu M;Zhang J
积雪草酸（AA）是存在于积雪草中的三萜类化合物之一，并且已显示其能够减轻肝纤维化。在本研究中，我们研究了AA对单侧输尿管梗阻（UUO）小鼠肾小管间质纤维化的影响。将小鼠随机分为5组（n=5/组）：假手术组（Sh）、UUO+溶剂处理组（UUO+V）、UUO +1 mg/kg体重AA处理组（UUO+A1）、UUO +4 mg/kg体重AA处理组（UUO+A2）和UUO +16 mg/kg体重AA处理组（UUO+A3）。从手术后第二天开始，每天用AA经口灌胃治疗小鼠，持续6天。第七天，处死小鼠进行检查。在给予溶剂的小鼠的肾皮质中观察到肾小管损伤，而观察到高剂量的AA对肾小管损伤产生显著的抑制作用。输尿管结扎可诱导间质纤维化、α-平滑肌肌动蛋白（SMA）和转化生长因子（TGF）-β1表达增加以及Smad 2/3磷酸化，但中、高剂量AA可消除这些作用。这些结果表明，AA可能通过减少由Smad依赖性TGF-β1信号传导介导的肾小管损伤、成纤维细胞活化和细胞外基质（ECM）积累来改善肾小管间质纤维化。
Asiatic acid (AA) is one of the triterpenoid compounds present in Centella asiatica and it has been shown to be capable of attenuating liver fibrosis. In the present study, we investigated the effects of AA on renal tubulointerstitial fibrosis in mice with unilateral ureteral obstruction (UUO). Mice were divided randomly into five groups (n=5 per group): the sham-surgery (Sh), UUO plus vehicle treatment (UUO+V), UUO plus 1 mg/kg body weight AA treatment (UUO+A1), UUO plus 4 mg/kg body weight AA treatment (UUO+A2) and UUO plus 16 mg/kg body weight AA treatment (UUO+A3) groups. The mice were treated with AA daily by oral gavage from the day subsequent to surgery for six days. On the seventh day, the mice were sacrificed for examination. Tubular injury was observed in the renal cortex of the mice administered the vehicle, while high doses of AA were observed to exert a significant suppressive effect on tubular injury. Interstitial fibrosis, increased expression of α-smooth muscle actin (SMA) and transforming growth factor (TGF)-β1 and phosphorylation of Smad2/3 were induced by ureteral ligation; however these effects were abrogated by intermediate and high doses of AA. These results suggest that AA may ameliorate tubulointerstitial fibrosis by reducing tubular injury, fibroblast activation and extracellular matrix (ECM) accumulation mediated by Smad-dependent TGF-β1 signaling.
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亚洲酸通过在体内和体外阻断TGF-β/SMAD信号传导来抑制肝纤维化。
影响因子：
3.7
作者：
Tang LX;He RH;Yang G;Tan JJ;Zhou L;Meng XM;Huang XR;Lan HY
通讯作者：
Lan HY
影响因子：
8.3
作者：
Lee KY;Bae ON;Serfozo K;Hejabian S;Moussa A;Reeves M;Rumbeiha W;Fitzgerald SD;Stein G;Baek SH;Goudreau J;Kassab M;Majid A
通讯作者：
Majid A
影响因子：
3.3
作者：
Gewin L;Zent R
通讯作者：
Zent R
影响因子：
9.2
作者：
Lan HY
通讯作者：
Lan HY
影响因子：
3.7
作者：
Kavitha CV;Agarwal C;Agarwal R;Deep G
通讯作者：
Deep G