Photoaffinity labeling of the hemolymph juvenile hormone binding protein of Manduca sexta.
Photoaffinity labeling of the hemolymph juvenile hormone binding protein of Manduca sexta.
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烟草天蛾血淋巴保幼激素结合蛋白的光亲和标记。
DOI：
10.1021/bi00321a060
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Gilbert,LI
中科院分区：
文献类型：
--
作者：
Koeppe,JK;Prestwich,GD;Brown,JJ;Goodman,WG;Kovalick,GE;Briers,T;Pak,MD;Gilbert,LI
John K. Koeppe,* Glenn D. Prestwich, John J. Brown, Walter G. Goodman, Gae E. Kovalick, Tony Briers, Michael D. Pak, and Lawrence I. Gilbert abstract: A synthetic analogue of the insect juvenile hormone (JH) III, 10, 1 l-epoxy [10-3H] farnesyl diazoacetate ([3H]-EFDA), binds to several proteins in a partially purified preparation of hemolymph protein from fourth instar larvae of Manduca sexta when irradiated with UV light. Approximately 80% of this binding could be inhibited by the addition of excess unlabeled JH I. To compare the relative affinity of EFDA for the juvenile hormone binding protein (JHBP) with that of the various JH homologues, the ability of unlabeled EFDA and JH homologues to displace [3H] JH I from binding sites was measured. The relativeaffinities were EFDA> JH I> JH II> JH III. When Scatchard analysis of the binding of [3H] EFDA or [3H] JH I to the larvalJHBP was performed, an estimated apparent KD of 4.5 X 10~ 8 M was found for EFDA, whereas for JH I a slightly higher KD of 8.8 X 10 “8M was calculated. To determine if [3H] EFDA bound at the JH I binding site, displacement of [3H] JH I from the JHBP complex with unlabeled JH I, JH II, and JH III was compared to the displacement of [3H] EFDA with the same homologues. The results demonstrated that the photoaffinity label bound covalently at the JH I binding site on the hemolymph binding protein of Manduca sexta. Fluorescence autoradiography of [3H] EFDA photoaffinity labeled proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that [3H] EFDA bound covalently to two major proteins in the absence of JHI. The presence of JH I prevented the binding of [3H] EFDA to one protein having a molecular weight of approximately 32 000, a molecular weight similar to that of the larval JHBPof Manducahemolymph.
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四龄烟草天蛾血淋巴中保幼激素结合蛋白的纯化和表征
影响因子：
4.1
作者：
W. Goodman;P. O'Hern;R. Zaugg;L. Gilbert
通讯作者：
L. Gilbert
烟草天蛾（Manduca sexta）血淋巴中的保幼激素特异性酯酶。
影响因子：
2.2
作者：
L. L. Sanburg;Karl J. Kramer;F. Kézdy;John H. Law
通讯作者：
John H. Law
昆虫保幼激素结合蛋白的光亲和标签。
影响因子：
2.1
作者：
G. Prestwich;A. Singh;J. F. Carvalho;John K.K. Oeppe;G. Kovalick;E. S. Chang
通讯作者：
E. S. Chang
马德拉白蚁保幼激素结合蛋白的测定与鉴定
影响因子：
4.1
作者：
G. Kovalick;J. Koeppe
通讯作者：
J. Koeppe
DOI：
10.1073/pnas.71.2.493
发表时间：
1974-01-01
影响因子：
11.1
作者：
KRAMER, KJ;SANBURG, LL;LAW, JH
通讯作者：
LAW, JH