Rapid and practical DNA isolation from Trichomonas vaginalis and other nuclease-rich protozoa.
Rapid and practical DNA isolation from Trichomonas vaginalis and other nuclease-rich protozoa.
复制标题
从阴道毛滴虫和其他富含核酸酶的原生动物中快速、实用地分离 DNA。
DOI:
10.1016/0166-6851(92)90212-3
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发表时间:
1992
影响因子:
1.5
通讯作者:
Krieger,JN
中科院分区:
文献类型:
--
作者:
Riley,DE;Krieger,JN
A method for the isolation of DNA fromT. vaginaliswhich uses DEPC (diethyl pyrocarbonate) and Triton X-100 is described. DNA isolated in this way cut well with restriction enzymes and reacted with T4 DNA ligase. It is considered that this method is effective because of strong nuclease inhibition by DEPC combined with a fractionating effect of centrifugation in 1% (v/v) Triton X-100 that separates the nuclei from the bulk of the nuclease activity.
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DOI:
10.1111/j.1550-7408.1973.tb06021.x
发表时间:
1973
期刊:
The Journal of protozoology
影响因子:
--
作者:
B. Honigberg;F. Mohn
通讯作者:
F. Mohn
DOI:
10.1073/pnas.87.16.6097
发表时间:
1990
影响因子:
11.1
作者:
Johnson,PJ;d'Oliveira,CE;Gorrell,TE;Müller,M
通讯作者:
Müller,M
DOI:
10.1093/infdis/152.5.979
发表时间:
1985
期刊:
The Journal of infectious diseases
影响因子:
--
作者:
Krieger,JN;Holmes,KK;Spence,MR;Rein,MF;McCormack,WM;Tam,MR
通讯作者:
Tam,MR
DOI:
--
发表时间:
1983
期刊:
The Journal of parasitology
影响因子:
--
作者:
Turner,G;Muller,M
通讯作者:
Muller,M
影响因子:
1.5
作者:
Wang,AL;Wang,CC
通讯作者:
Wang,CC