Inhibition of prostate cancer growth and metastasis using small interference RNA specific for minichromosome complex maintenance component 7.
Inhibition of prostate cancer growth and metastasis using small interference RNA specific for minichromosome complex maintenance component 7.
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DOI：
10.1038/cgt.2010.25
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发表时间：
2010-10
影响因子：
6.4
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中科院分区：
文献类型：
--
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微染色体复合体维护组件7(MCM7)是DNA复制许可的关键组件。MCM7的扩增和过表达导致前列腺癌的高转移率。最近的研究表明，MCM7基因组编码一个可能的包括MCM7癌基因在内的超级癌基因簇和一个下调几个关键抑癌基因表达的miRNA簇。在本研究中，我们构建了一个结构性表达MCM7特异性siRNA的载体。将该载体导入前列腺癌PC3或Du145细胞，可使MCM7的表达降低80%。该载体抑制DNA合成，并使这些癌细胞生长停滞。将SCID小鼠移植入PC3或Du145肿瘤，通过尾静脉注射聚乙烯亚胺(PEI)对其进行治疗。与对照组相比，动物的肿瘤体积显著减小，转移更少，生存率更高。因此，使用siRNA方法干预MCM7的表达可能为雄激素耐药前列腺癌的治疗带来希望。
Minichromosome complex maintenance component 7 (MCM7) is a critical component of DNA replication licensing. Amplification and overexpression of MCM7 leads to high rate of prostate cancer metastasis. Recent studies indicate that MCM7 genome encodes a putative “super-oncogene” cluster including MCM7 oncogene and a miRNA cluster that knocks down the expression of several critical tumor suppressor genes. In this study, we constructed a vector that constitutively expresses siRNA specific for MCM7. Introduction of this vector into prostate cancer cell lines PC3 or Du145 decreases the expression of MCM7 by 80%. The vector inhibits DNA synthesis and generates growth arrest of these cancer cells. SCID mice were xenografted PC3 or Du145 tumors, and subsequently treated with this vector through tail vein injection with polyethylenimine (PEI). The animals had dramatically smaller tumor volume, less metastasis and better survival rate in comparison with the controls. As a result, intervention of MCM7 expression using siRNA approach may hold the promise for treating androgen refractory prostate cancer.
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影响因子：
8
作者：
Ren, B;Yu, G;Luo, JH
通讯作者：
Luo, JH
影响因子：
19
作者：
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通讯作者：
Cheung, ANY
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作者：
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