Interferometric methods for label-free molecular interaction studies.
Interferometric methods for label-free molecular interaction studies.
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DOI：
10.1021/ac202812h
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发表时间：
2012-01-17
影响因子：
7.4
通讯作者：
Bornhop, Darryl J.
中科院分区：
文献类型：
--
作者：
Kussrow, Amanda;Enders, Carolyn S.;Bornhop, Darryl J.
DNA相互作用。在生物传感的前沿领域，已经开发了许多干涉测量系统，它们在测量分子相互作用方面比传统方法具有显著的优势。特别是，干涉计量学使检测和研究结合事件成为可能，而无需使用昂贵和破坏性的标记。在过去的几年里，利用干涉测量技术的最新进展取得了显著的进步。例如，通过使用纳米线光栅和多个几何形状，衍射光学得到了改进，从而增强了多路复用并增加了仪器的动态范围。双极化方面的创新展示了在测量动力学的同时捕获结合伙伴的结构信息的能力。诸如微环谐振器之类的波导型微谐振器在保持简单且廉价的多路传输格式的同时显示出了精致的灵敏度。新的生物传感技术也出现了，例如背向散射干涉法，它独特地提供了测量自由溶液中结合作用的额外优势，同时仍然使用少量样品。
DNA interactions. On the biosensing frontier, a number of interferometric systems have been developed which offer significant advantages over traditional methods for measuring molecular interactions. In particular, interferometry enables the detection and study of binding events without the use of expensive and disruptive labels. Recent advancements in technologies utilizing interferometry have made significant improvements in the last several years. For example, diffraction optics has been improved through the use of nanowire gratings and multiple geometries, resulting in enhanced multiplexing and an increased instrument dynamic range. Innovations in dual polarization have demonstrated the ability to measure kinetics while simultaneously capturing structural information about the binding partners. Waveguiding microresonators, such as the microring resonator, have shown exquisite sensitivities while maintaining a straightforward and inexpensive multiplex format. New biosensing technologies have also come forward, such as backscattering interferometry which uniquely offers the additional advantage of measuring binding interactions in free solution while still using small amounts of sample.
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DOI：
10.1098/rsta.2001.0977
发表时间：
2002-05-15
影响因子：
5
作者：
Baldwin, JE;Haniff, CA
通讯作者：
Haniff, CA
影响因子：
3.9
作者：
Elkashef, H
通讯作者：
Elkashef, H
影响因子：
15
作者：
Dancil, KPS;Greiner, DP;Sailor, MJ
通讯作者：
Sailor, MJ
影响因子：
2.9
作者：
Abdiche, Yasmina;Malashock, Dan;Pons, Jaurne
通讯作者：
Pons, Jaurne
影响因子：
4.3
作者：
Birkert, O;Gauglitz, G
通讯作者：
Gauglitz, G