Derivation of clones close to met by preparative field inversion gel electrophoresis.
Derivation of clones close to met by preparative field inversion gel electrophoresis.
复制标题
通过制备型场反转凝胶电泳衍生出接近满足的克隆。
作者：
F. Michiels;M. Burmeister;H. Lehrach
通过突变鉴定基因的分子分析是哺乳动物遗传学的一个主要问题。为了实现这一目标，制备场反转凝胶电泳（FIGE）被用于从遗传连锁标记的环境中选择性地分离克隆，并选择这些克隆中的一个子集，其中包含哺乳动物DNA中罕见的特定限制性位点旁边的序列。这种方法已被用于生成一个高度富集与囊性纤维化标记物密切相关的序列的文库。从含有met序列的Not I限制性内切片段末端衍生的一个克隆进行了详细分析，并在metD序列的300千碱基对5'的长范围图中定位。
The molecular analysis of genes identified by mutations is a major problem in mammalian genetics. As a step toward this goal, preparative field inversion gel electrophoresis (FIGE) was used to selectively isolate clones from the environment of genetically linked markers, and to select a subset of these clones containing sequences next to specific restriction sites rare in mammalian DNA. This approach has been used to generate a library highly enriched in sequences closely linked to the cystic fibrosis marker met. One clone derived from the end of a Not I restriction fragment containing the met sequence was analyzed in detail and localized within a long range map to a position 300 kilobase pairs 5' of the metD sequence.
囊性纤维化与两个紧密相连的 DNA 标记的联系：一项合作研究的联合报告。
影响因子：
9.8
作者：
Beaudet,A;Bowcock,A;Buchwald,M;Cavalli-Sforza,L;Farrall,M;King,MC;Klinger,K;Lalouel,JM;Lathrop,G;Naylor,S
通讯作者：
Naylor,S
通过体内重组和选择从噬菌体文库中纯化基因组序列。
影响因子：
14.9
作者：
Seed,B
通讯作者：
Seed,B