Cryo-electron microscopy--a primer for the non-microscopist.
Cryo-electron microscopy--a primer for the non-microscopist.
复制标题
DOI：
10.1111/febs.12078
复制
发表时间：
2013-01
期刊：
影响因子：
--
通讯作者：
Subramaniam S
中科院分区：
文献类型：
--
作者：
Milne JL;Borgnia MJ;Bartesaghi A;Tran EE;Earl LA;Schauder DM;Lengyel J;Pierson J;Patwardhan A;Subramaniam S
冷冻电子显微镜（cryo-EM）正日益成为以分子分辨率研究细胞、病毒和蛋白质组装体结构的主流技术。显微镜设计和成像硬件的最新发展，加上增强的图像处理和自动化能力，似乎有望进一步提高冷冻EM方法的有效性。这些发展有望提高自动化的速度和程度，并提高可以实现的分辨率，使这项技术能够确定各种各样的生物结构。此外，已建立的结构测定模式，如X射线晶体学和核磁共振光谱学，通常与cryo-EM密度图相结合，以实现复杂的动态分子组装的原子分辨率模型。在这篇评论中，这是针对读者谁不是专家在冷冻EM方法，我们提供了一个概述的新兴主题，在应用这项技术的生物学和医学的各种问题的调查。我们讨论了这些方法被用来研究大分子组装体的结构，这些组装体的大小从整个细胞到小蛋白质不等。最后，我们描述了如何将冷冻EM获得的结构信息保存和存档在可公开访问的数据库中。
Cryo-electron microscopy (cryo-EM) is increasingly becoming a mainstream technology for studying the architecture of cells, viruses and protein assemblies at molecular resolution. Recent developments in microscope design and imaging hardware, paired with enhanced image processing and automation capabilities, appear poised to further advance the effectiveness of cryo-EM methods. These developments promise to increase the speed and extent of automation and to improve the resolutions that can be achieved, rendering this technology capable of determining a wide variety of biological structures. Additionally, established modalities for structure determination, such as X-ray crystallography and nuclear magnetic resonance spectroscopy, are being routinely integrated with cryo-EM density maps to achieve atomic-resolution models of complex, dynamic molecular assemblies. In this review, which is directed towards readers who are not experts in cryo-EM methodology, we provide an overview of emerging themes in the application of this technology to the investigation of diverse questions in biology and medicine. We discuss the ways in which these methods are being used to study structures of macromolecular assemblies that range in size from whole cells to small proteins. Finally, we include a description of how the structural information obtained by cryo-EM is deposited and archived in a publicly accessible database.
登录
查看更多内容
影响因子：
3
作者：
Ahmed, Aqeel;Whitford, Paul C.;Sanbonmatsu, Karissa Y.;Tama, Florence
通讯作者：
Tama, Florence
影响因子：
48
作者：
Beck, Martin;Malmstroem, Johan A.;Lange, Vinzenz;Schmidt, Alexander;Deutsch, Eric W.;Aebersold, Ruedi
通讯作者：
Aebersold, Ruedi
影响因子：
64.8
作者：
DEROSIER, DJ;KLUG, A
通讯作者：
KLUG, A
影响因子：
64.8
作者：
Gavin, AC;Bösche, M;Superti-Furga, G
通讯作者：
Superti-Furga, G
影响因子：
3.2
作者：
Butan, Carmen;Hartnell, Lisa M.;Milne, Jacqueline L. S.
通讯作者：
Milne, Jacqueline L. S.