Considerations for Biotribometers: Cells, Gels, and Tissues
Considerations for Biotribometers: Cells, Gels, and Tissues
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生物摩擦磨损测试仪的注意事项：细胞、凝胶和组织
DOI：
10.1007/s11249-018-1094-y
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发表时间：
2018
影响因子：
3.2
通讯作者：
A. Pitenis
中科院分区：
文献类型：
--
作者：
J. Urueña;Samuel M. Hart;Derek L. Hood;Eric O. McGhee;Sean R. Niemi;K. Schulze;P. Levings;W. Sawyer;A. Pitenis
生物材料（如细胞和组织）的摩擦学评价对实验者来说既是机遇又是挑战。当与活体材料一起工作时，在体外或体内测试期间保持体内平衡通常需要适当控制环境、选择测试时间和持续时间、施加的载荷和剪切应力。这份手稿提供了大量的背景和设计信息，用于开发的微摩擦计，已被修改，以执行生物摩擦学测量体外。本手稿的重点是单层哺乳动物细胞，并使用一系列数量级计算为未来的仪器设计和考虑提供信息，包括：滑动速度范围从10 nm/s到100 mm/s，接触压力小于6 kPa，温度约37 °C，接触面积约为1，000 µm2。这些生物摩擦学仪器的设计和开发使原位荧光显微镜成为可能，并允许进行统计学上显著的基因表达分析，如定量逆转录聚合酶链反应（RT-qPCR）和酶联免疫吸附测定（ELISA）。
Tribological evaluation of biological materials, such as cells and tissues, present both opportunities and challenges to experimentalists. When working with living materials, maintaining homeostasis during testing in vitro or in vivo often requires appropriate control of the environment, selection of the testing time and duration, applied loads, and shear stresses. This manuscript provides much of the background and design information used in the development of a microtribometer that has been modified to perform biotribology measurements in vitro. The focus of this manuscript is on mammalian cells in monolayer, and a series of order-of-magnitude calculations are used to inform future instrument designs and considerations, including: sliding speed ranges from 10 nm/s to 100 mm/s, contact pressures less than 6 kPa, temperature ~ 37 °C, and contact areas on the order of 1,000’s of µm2. The design and development of these biotribology instruments enable in situ fluorescence microscopy and allow for statistically significant gene expression analyses such as quantitative reverse-transcription polymerase chain-reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA).
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影响因子：
13.6
作者：
Bhattacharjee T;Zehnder SM;Rowe KG;Jain S;Nixon RM;Sawyer WG;Angelini TE
通讯作者：
Angelini TE
影响因子：
13.6
作者：
O'Bryan CS;Bhattacharjee T;Hart S;Kabb CP;Schulze KD;Chilakala I;Sumerlin BS;Sawyer WG;Angelini TE
通讯作者：
Angelini TE
DOI：
10.1016/j.jtos.2017.12.002
发表时间：
2018-04
期刊：
The ocular surface
影响因子：
--
作者：
Guoting Qin;Hasna Baidouri;A. Glasser;VijayKrishna Raghunathan;C. Morris;I. Maltseva;A. McDermott
通讯作者：
Guoting Qin;Hasna Baidouri;A. Glasser;VijayKrishna Raghunathan;C. Morris;I. Maltseva;A. McDermott
影响因子：
5
作者：
O'Bryan, Christopher S.;Bhattacharjee, Tapomoy;Angelini, Thomas E.
通讯作者：
Angelini, Thomas E.