Monitoring SARS-CoV-2 in municipal wastewater to evaluate the success of lockdown measures for controlling COVID-19 in the UK.
Monitoring SARS-CoV-2 in municipal wastewater to evaluate the success of lockdown measures for controlling COVID-19 in the UK.
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DOI：
10.1016/j.watres.2021.117214
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发表时间：
2021-07-15
期刊：
影响因子：
12.8
通讯作者：
Jones DL
中科院分区：
文献类型：
--
作者：
Hillary LS;Farkas K;Maher KH;Lucaci A;Thorpe J;Distaso MA;Gaze WH;Paterson S;Burke T;Connor TR;McDonald JE;Malham SK;Jones DL
SARS-CoV-2及其引发的COVID-19大流行是近期对人类健康、福祉和经济增长的最大威胁之一。基于废水的人类病毒流行病学（WBE）可成为人口规模监测SARS-CoV-2流行和流行病学的有用工具，以帮助防止该疾病的进一步传播，特别是在城市中心。在这里，我们通过q(RT-)PCR和病毒基因组测序，对英国六个主要城市中心（总人口相当于300万）污水中SARS-CoV-2 RNA的流行率进行了纵向分析（2020年3月至7月）。我们的研究结果表明，SARS-CoV-2 RNA水平通常与大城市中心社区内记录的临床病例丰度相关，在实施封锁措施后，SARS-CoV-2 RNA丰度显着下降。在COVID-19确诊病例数较低的地区，这种关联强度较弱。此外，对来自废水的SARS-CoV-2的序列分析表明，在我们采样点覆盖的当地人群中存在多个遗传上不同的聚集性，并且在废水中观察到的遗传变异反映了在临床诊断实验室检测的病例的同期样本中观察到的相似snp。我们展示了如何将WBE用于社区层面的SARS-CoV-2和其他病毒流行情况的检测和跟踪，并为公共卫生政策决策提供信息。不过，为了将WBE数据充分整合到疫情监测中，需要更好地了解影响废水中SARS-CoV-2 RNA浓度的因素。综上所述，本研究结果为常规WBE监测人群中流行的SARS-CoV-2和其他人致病性病毒以及评估疾病控制措施的有效性提供了支持。
SARS-CoV-2 and the resulting COVID-19 pandemic represents one of the greatest recent threats to human health, wellbeing and economic growth. Wastewater-based epidemiology (WBE) of human viruses can be a useful tool for population-scale monitoring of SARS-CoV-2 prevalence and epidemiology to help prevent further spread of the disease, particularly within urban centres. Here, we present a longitudinal analysis (March–July 2020) of SARS-CoV-2 RNA prevalence in sewage across six major urban centres in the UK (total population equivalent 3 million) by q(RT-)PCR and viral genome sequencing. Our results demonstrate that levels of SARS-CoV-2 RNA generally correlated with the abundance of clinical cases recorded within the community in large urban centres, with a marked decline in SARS-CoV-2 RNA abundance following the implementation of lockdown measures. The strength of this association was weaker in areas with lower confirmed COVID-19 case numbers. Further, sequence analysis of SARS-CoV-2 from wastewater suggested that multiple genetically distinct clusters were co-circulating in the local populations covered by our sample sites, and that the genetic variants observed in wastewater reflected similar SNPs observed in contemporaneous samples from cases tested in clinical diagnostic laboratories. We demonstrate how WBE can be used for both community-level detection and tracking of SARS-CoV-2 and other virus’ prevalence, and can inform public health policy decisions. Although, greater understanding of the factors that affect SARS-CoV-2 RNA concentration in wastewater are needed for the full integration of WBE data into outbreak surveillance. In conclusion, our results lend support to the use of routine WBE for monitoring of SARS-CoV-2 and other human pathogenic viruses circulating in the population and assessment of the effectiveness of disease control measures.
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影响因子：
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