A functional TOC complex contributes to gravity signal transduction in Arabidopsis.
A functional TOC complex contributes to gravity signal transduction in Arabidopsis.
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DOI：
10.3389/fpls.2014.00148
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发表时间：
2014
影响因子：
5.6
通讯作者：
Masson PH
中科院分区：
文献类型：
--
作者：
Strohm AK;Barrett-Wilt GA;Masson PH
虽然质体沉降长期以来一直被认为是重要的植物的重力感知，它最近被证明，质体在向重力性发挥额外的功能。叶绿体外被膜（TOC）复合体上的易位子将核编码的蛋白质转运到质体中，该复合体的受体Toc132先前被假设通过直接充当重力信号转导器或间接介导重力信号转导器的质体定位来促进向重力性。在这里，我们表明，在多个基因编码TOC复杂的组件的突变影响向重力性在遗传致敏的背景下，Toc132的细胞质酸性结构域是不需要参与这一过程。此外，突变TOC132增强重力缺陷的突变体的淀粉体缺乏淀粉。最后，我们表明，几个核编码的根蛋白的水平改变toc132突变体。这些数据表明，TOC复合物间接介导的拟南芥中的重力信号转导和支持的想法，质体参与向重力性，不仅通过他们的沉淀能力，但也作为信号转导机制的一部分。
Although plastid sedimentation has long been recognized as important for a plant's perception of gravity, it was recently shown that plastids play an additional function in gravitropism. The Translocon at the Outer envelope membrane of Chloroplasts (TOC) complex transports nuclear-encoded proteins into plastids, and a receptor of this complex, Toc132, was previously hypothesized to contribute to gravitropism either by directly functioning as a gravity signal transducer or by indirectly mediating the plastid localization of a gravity signal transducer. Here we show that mutations in multiple genes encoding TOC complex components affect gravitropism in a genetically sensitized background and that the cytoplasmic acidic domain of Toc132 is not required for its involvement in this process. Furthermore, mutations in TOC132 enhance the gravitropic defect of a mutant whose amyloplasts lack starch. Finally, we show that the levels of several nuclear-encoded root proteins are altered in toc132 mutants. These data suggest that the TOC complex indirectly mediates gravity signal transduction in Arabidopsis and support the idea that plastids are involved in gravitropism not only through their ability to sediment but also as part of the signal transduction mechanism.
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