CRISPR/Cas9-Mediated Multiplex Genome Editing of JAGGED Gene in Brassica napus L.
CRISPR/Cas9-Mediated Multiplex Genome Editing of JAGGED Gene in Brassica napus L.
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CRISPR/Cas9介导的甘蓝型油菜JAGGED基因的多重基因组编辑。
DOI：
10.3390/biom9110725
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发表时间：
2019-11
期刊：
影响因子：
5.5
通讯作者：
Huqiong
中科院分区：
文献类型：
--
作者：
Zaman Q U;Chu Wen;Hao mengyu;Shi yuqin;Sun mengdan;Sang shifei;Mei desheng;Cheng hongtao;Liu jia;Li Chao;Huqiong
抗落荚性是实现油菜高产的重要组成部分，是油菜多倍体栽培的重要目标。已有研究表明，拟南芥JAGGED （JAG）基因是参与果实开裂调控网络的关键因子。但其在油菜抗碎荚性中的作用尚不清楚。在本研究中，利用CRISPR/Cas9系统对5个同源物（BnJAG）进行多重基因组编辑。A02 BnJAG。二氧化碳,BnJAG。C06 BnJAG。A07和BnJAG。A08)的JAG基因。所有同源基因的敲除突变极大地影响了组织荚果形状和大小的侧面器官的发育。荚果的圆柱形体由许多未分化的细胞组成，如愈伤组织，没有明显的瓣、复瓣、隔和瓣缘。假种子被分成两半，其中有一层不完整的细胞（可能是隔膜）将未分化的细胞分开。这些突变体不能为后代产生任何有效的种子。然而，在一个突变系中发现只有一个BnJAG。JAG基因a08 - nub样平行体发生突变。BnJAG的基因敲除突变。A08-NUB基因引起了豆荚开裂区的显著变化。突变体的复顶区显著增加，细胞大小增大，果实凹凸不平，长度较野生型缩短。与野生型相比，较高的阀节面积可能使JAG突变体抗荚果破碎的能力提高了2倍。本研究结果为了解甘蓝型油菜果实JAG基因的突变提供了基础，并为其他芸苔科植物的JAG基因突变提供了思路。
Pod shattering resistance is an essential component to achieving a high yield, which is a substantial objective in polyploid rapeseed cultivation. Previous studies have suggested that the Arabidopsis JAGGED (JAG) gene is a key factor implicated in the regulatory web of dehiscence fruit. However, its role in controlling pod shattering resistance in oilseed rape is still unknown. In this study, multiplex genome editing was carried out by the CRISPR/Cas9 system on five homoeologs (BnJAG.A02, BnJAG.C02, BnJAG.C06, BnJAG.A07, and BnJAG.A08) of the JAG gene. Knockout mutagenesis of all homoeologs drastically affected the development of the lateral organs in organizing pod shape and size. The cylindrical body of the pod comprised a number of undifferentiated cells like a callus, without distinctive valves, replum, septum, and valve margins. Pseudoseeds were produced, which were divided into two halves with an incomplete layer of cells (probably septum) that separated the undifferentiated cells. These mutants were not capable of generating any productive seeds for further generations. However, one mutant line was identified in which only a BnJAG.A08-NUB-Like paralog of the JAG gene was mutated. Knockout mutagenesis in BnJAG.A08-NUB gene caused significant changes in the pod dehiscence zone. The replum region of the mutant was increased to a great extent, resulting in enlarged cell size, bumpy fruit, and reduced length compared with the wild type. A higher replum–valve joint area may have increased the resistance to pod shattering by ~2-fold in JAG mutants compared with wild type. Our results offer a basis for understanding variations in Brassica napus fruit by mutating JAG genes and providing a way forward for other Brassicaceae species.
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一种高效的 CRISPR/Cas9 平台，用于快速生成异源四倍体油菜中多基因同源物的同时诱变
影响因子：
5.6
作者：
Li C;Hao M;Wang W;Wang H;Chen F;Chu W;Zhang B;Mei D;Cheng H;Hu Q
通讯作者：
Hu Q
影响因子：
6.6
作者：
Hu, Qiong;Hua, Wei;Wang, Hanzhong
通讯作者：
Wang, Hanzhong
影响因子：
7.2
作者：
Horigome, Ayako;Nagasawa, Nobuhiro;Nagato, Yasuo
通讯作者：
Nagato, Yasuo
影响因子：
4.5
作者：
González-Reig S;Ripoll JJ;Vera A;Yanofsky MF;Martínez-Laborda A
通讯作者：
Martínez-Laborda A
DOI：
10.1111/nph.14282
发表时间：
2017-10
期刊：
The New phytologist
影响因子：
--
作者：
Ya Min;E. Kramer
通讯作者：
Ya Min;E. Kramer