Detection of collagenase mRNA in odontoclasts of bovine root-resorbing tissue by In situ hybridization
Detection of collagenase mRNA in odontoclasts of bovine root-resorbing tissue by In situ hybridization
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原位杂交检测牛根吸收组织破牙细胞中胶原酶mRNA
DOI：
10.1007/bf00296659
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发表时间：
1993
影响因子：
4.2
通讯作者：
S. Sasaki
中科院分区：
文献类型：
--
作者：
Takeshi Okamura;H. Shimokawa;Y. Takagi;H. Ono;S. Sasaki
破牙细胞在形态上与破骨细胞相似，被认为在乳牙的牙根吸收中起重要作用。在牙根吸收组织中检测到较高的胶原酶活性。为了鉴定胶原酶产生细胞及其在乳牙牙根吸收中的作用，用地高辛标记的非放射性RNA探针原位杂交了牛牙牙根吸收组织切片中的胶原酶mRNA。除巨噬细胞、成纤维细胞、破牙本质细胞和成牙骨质细胞外，破牙本质细胞中也有胶原酶mRNA的表达。多核破牙细胞显示出较强的抗酒石酸酸性磷酸酶(TRAP)活性。用原位杂交法检测牙根吸收组织中白介素1(IL-1)的表达。IL-1在破牙细胞、成纤维细胞和巨噬细胞中均有表达，提示IL-1可能是促进牙根吸收的重要因素。这些结果提示，破牙细胞产生的胶原酶可能在牙本质胶原降解过程中起关键作用。
SummaryThe odontoclast, which is morphologically similar to osteoclast, is thought to play a major role in root resorption of deciduous teeth. High collagenolytic activity has been detected in the root resorbing tissue. In order to identify collagenase-producing cells and the role of collagenase in root resorption of deciduous tooth, in situ hybridization of collagenase mRNA in bovine root-resorbing tissue sections was performed using a digoxigenin-labeled, nonradioactive RNA probe. Collagenase mRNA expression was clearly observed in odontoclasts in addition to the macrophages, fibroblasts, odontoclasts, and cementoblasts. Multinuclear odontoclasts showed intense tartrate-resistant acid phosphatase (TRAP) activity. We also examined interleukin-1 (IL-1) mRNA expression in the root-resorbing tissue by in sity hybridization. IL-1 transcripts were found to be expressed in odontoclasts, fibroblasts, and macrophages suggesting that IL-1 might be an important factor for promoting root resorption. These results suggest that the collagenase produced by odontoclasts may play a key role in dentin collagen degradation in root resorption.
影响因子：
2.7
作者：
COX, KH;DELEON, DV;ANGERER, RC
通讯作者：
ANGERER, RC
胶原酶在组织培养中吸收骨的生化和免疫组织化学研究。
影响因子：
3.5
作者：
Sakamoto,S;Sakamoto,M
通讯作者：
Sakamoto,M