Comparison of isothiocyanate metabolite levels and histone deacetylase activity in human subjects consuming broccoli sprouts or broccoli supplement.
Comparison of isothiocyanate metabolite levels and histone deacetylase activity in human subjects consuming broccoli sprouts or broccoli supplement.
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DOI：
10.1021/jf202887c
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发表时间：
2011-10-26
影响因子：
6.1
通讯作者：
Ho E
中科院分区：
文献类型：
--
作者：
Clarke JD;Riedl K;Bella D;Schwartz SJ;Stevens JF;Ho E
增加十字花科蔬菜的消费，如西兰花，可以降低患各种癌症的风险。黑芥子酶是将西兰花中的芥子油苷转化为具有生物活性的异硫氰酸酯所必需的。我们评估了食用西兰花芽或西兰花补充剂（无黑芥子酶）的健康受试者的异硫氰酸盐排泄谱，其中硫代葡萄糖苷含量相等。尿液中的代谢物的两个主要的异硫氰酸酯，萝卜硫素和芥酸，测定液相色谱串联质谱法。与食用补充剂的受试者相比，食用西兰花芽的受试者的萝卜硫素和芥酸的峰值排泄更高，发生得更早。在两组中均观察到尿萝卜硫素与芥酸代谢物的比值的受试者依赖性变化，表明萝卜硫素转化为芥酸。组蛋白去乙酰化酶活性较低，观察到外周血单核细胞中，只有在受试者消耗芽。总之，新鲜的西兰花芽不同于西兰花补充剂在人类受试者中的异硫氰酸酯排泄和生物活性方面。
Increased consumption of cruciferous vegetables such as broccoli may reduce the risk of various cancers. Myrosinase is required to convert dietary glucosinolates from broccoli into bioactive isothiocyanates. We evaluated isothiocyanate excretion profiles in healthy subjects who consumed broccoli sprouts or broccoli supplement (no myrosinase) with equivalent glucosinolate content. Urinary metabolites of two major isothiocyanates, sulforaphane and erucin, were measured by liquid chromatography coupled with tandem mass spectrometry. Peak excretion of sulforaphane and erucin was higher and occurred sooner in subjects who consumed broccoli sprouts compared to subjects who consumed the supplement. A subject-dependent shift in the ratio of urinary sulforaphane to erucin metabolites was observed in both groups indicating conversion of sulforaphane to erucin. Lower histone deacetylase activity was observed in the peripheral blood mononuclear cells only in subjects consuming sprouts. In conclusion, fresh broccoli sprouts differ from broccoli supplements in regards to excretion of isothiocyanates and bioactivity in human subjects.
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