Solving Kinetic Equations for the Laser Flash Photolysis Experiment on Nitric Oxide Synthases: Effect of Conformational Dynamics on the Interdomain Electron Transfer.
Solving Kinetic Equations for the Laser Flash Photolysis Experiment on Nitric Oxide Synthases: Effect of Conformational Dynamics on the Interdomain Electron Transfer.
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DOI：
10.1021/acs.jpca.5b08414
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发表时间：
2015-11-12
期刊：
影响因子：
--
通讯作者：
Feng C
中科院分区：
文献类型：
--
作者：
Astashkin AV;Feng C
一氧化氮合酶 (NOS) 产生一氧化氮取决于黄素单核苷酸 (FMN) 和血红素结构域之间的结构域间电子转移 (IET)。尽管已经通过激光闪光光解（LFP）测量了各种NOS蛋白的IET速率，但迄今为止尚未对相关动力学方程进行严格分析。在这项工作中，我们提供了 LFP 方法背后的动力学方程的解析解。推导的表达式表明，整体 IET 速率受到构象动力学的显着影响，构象动力学决定 FMN 和血红素结构域之间对接复合物的形成和解离速率。我们表明，为了详细研究 NOS 酶上的电子转移，LFP 应与其他可以直接探测构象速率常数的光谱方法结合使用。讨论了所获得的分析表达式对于解释各种天然和修饰的 NOS 蛋白的 LFP 结果的影响。这项工作中推导的数学公式也应该适用于解释其他模块化氧化还原酶的 IET 动力学。
The production of nitric oxide by the nitric oxide synthase (NOS) enzyme depends on the interdomain electron transfer (IET) between the flavin mononucleotide (FMN) and heme domains. Although the rate of this IET has been measured by laser flash photolysis (LFP) for various NOS proteins, no rigorous analysis of the relevant kinetic equations was performed so far. In this work, we provide an analytical solution of the kinetic equations underlying the LFP approach. The derived expressions reveal that the bulk IET rate is significantly affected by the conformational dynamics that determines the formation and dissociation rates of the docking complex between the FMN and heme domains. We show that in order to informatively study the electron transfer across the NOS enzyme, LFP should be used in combination with other spectroscopic methods that could directly probe the conformational rate constants. The implications of the obtained analytical expressions for the interpretation of the LFP results from various native and modified NOS proteins are discussed. The mathematical formulae derived in this work should also be applicable for interpreting the IET kinetics in other modular redox enzymes.
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影响因子：
3.5
作者：
Li W;Fan W;Elmore BO;Feng C
通讯作者：
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DOI：
10.1016/j.bbapap.2011.08.004
发表时间：
2011-12-01
影响因子：
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作者：
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作者：
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