Chicken CH25H inhibits ALV-J replication by promoting cellular autophagy.
Chicken CH25H inhibits ALV-J replication by promoting cellular autophagy.
复制标题
鸡CH 25 H通过促进细胞自噬抑制ALV-J复制。
DOI：
10.3389/fimmu.2023.1093289
复制
发表时间：
2023
影响因子：
7.3
通讯作者：
Zhang, Xiquan
中科院分区：
文献类型：
--
作者：
Xie, Tingting;Feng, Min;Zhang, Xi;Li, Xiaoqi;Mo, Guodong;Shi, Meiqing;Zhang, Xiquan
自噬在宿主抗病毒防御中起着重要作用。J亚群禽白血病病毒（ALV-J）已被证明抑制自噬，同时促进病毒复制。然而，潜在的自噬机制尚不清楚。胆固醇25-羟化酶（CH 25 H）是一个保守的干扰素刺激基因，可将胆固醇转化为可溶性抗病毒因子25-羟基胆固醇（25 HC）。本研究进一步探讨了CH 25 H在鸡胚成纤维细胞系（DF 1）中抗ALV-J的自噬机制。我们的研究结果发现，CH 25 H的过表达和25 HC处理促进了自噬标志物微管相关蛋白1轻链3 II（LC 3 II）和自噬相关基因5（ATG 5）的表达，同时降低了自噬底物p62/SQSTM 1（p62）在ALV-J感染DF-1细胞中的表达。细胞自噬的诱导也降低ALV-J gp 85和p27的水平。另一方面，ALV-J感染抑制自噬标记蛋白LC 3 II表达。这些发现表明，CH 25 H诱导的自噬是一种有助于ALV-J复制抑制的宿主防御机制。特别是，CH 25 H与CHMP 4 B相互作用，通过促进自噬抑制DF-1细胞中的ALV-J感染，揭示了CH 25 H抑制ALV-J感染的新机制。虽然其潜在机制尚未完全了解，但CH 25 H和25 HC是第一个显示通过自噬抑制ALV-J感染的。
Autophagy plays an important role in host antiviral defense. The avian leukosis virus subgroup J (ALV-J) has been shown to inhibit autophagy while promoting viral replication. The underlying autophagic mechanisms, however, are unknown. Cholesterol 25-hydroxylase (CH25H) is a conserved interferon-stimulated gene, which converts cholesterol to a soluble antiviral factor, 25-hydroxycholesterol (25HC). In this study, we further investigated the autophagic mechanism of CH25H resistance to ALV-J in chicken embryonic fibroblast cell lines (DF1). Our results found that overexpression of CH25H and treatment with 25HC promoted the autophagic markers microtubule-associated protein 1 light chain 3 II (LC3II) and autophagy-related gene 5(ATG5), while decreased autophagy substrate p62/SQSTM1 (p62) expression in ALV-J infection DF-1 cells. Induction of cellular autophagy also reduces the levels of ALV-J gp85 and p27. ALV-J infection, on the other hand, suppresses autophagic marker protein LC3II expression. These findings suggest that CH25H-induced autophagy is a host defense mechanism that aids in ALV-J replication inhibition. In particular, CH25H interacts with CHMP4B and inhibits ALV-J infection in DF-1 cells by promoting autophagy, revealing a novel mechanism by which CH25H inhibits ALV-J infection. Although the underlying mechanisms are not completely understood, CH25H and 25HC are the first to show inhibiting ALV-J infection via autophagy.
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DOI：
10.1038/s41579-018-0003-6
发表时间：
2018-06
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Choi Y;Bowman JW;Jung JU
通讯作者：
Jung JU
影响因子：
4.8
作者：
Gunduz F;Aboulnasr FM;Chandra PK;Hazari S;Poat B;Baker DP;Balart LA;Dash S
通讯作者：
Dash S
DOI：
10.1083/jcb.201503023
发表时间：
2015-09-14
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kimura T;Jain A;Choi SW;Mandell MA;Schroder K;Johansen T;Deretic V
通讯作者：
Deretic V
影响因子：
4.8
作者：
Adnan, Mohammad;Malathi, Siddiqui Krishnamurthy
通讯作者：
Malathi, Siddiqui Krishnamurthy
影响因子：
7.3
作者：
Hayashi K;Taura M;Iwasaki A
通讯作者：
Iwasaki A