A type VI secretion system delivers a cell wall amidase to target bacterial competitors.
A type VI secretion system delivers a cell wall amidase to target bacterial competitors.
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VI 型分泌系统将细胞壁酰胺酶传递给目标细菌竞争者
DOI：
10.1111/mmi.14513
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发表时间：
2020-08
影响因子：
3.6
通讯作者：
Liang H
中科院分区：
文献类型：
--
作者：
Wang T;Hu Z;Du X;Shi Y;Dang J;Lee M;Hesek D;Mobashery S;Wu M;Liang H
人类病原体铜绿假单胞菌含有三种同源的锌蛋白酶，分别标记为amppd、AmpDh2和AmpDh3，它们可以翻动细胞壁和细胞壁衍生的多肽。AmpD是细胞质的，在细胞壁多肽的再循环中起作用，与抗生素耐药性有关。AmpDh2是一种质周可溶性酶，前者固定在外膜的内小叶上。我们在此记录了P. aeruginosa的VI型分泌系统II位点（H2-T6SS）在接触猎物细菌时将AmpDh3（但不是AmpD或AmpDh2）传递到周围质。AmpDh3水解被捕食细菌的细胞壁肽聚糖，导致其死亡，从而为铜绿假单胞菌在细菌竞争中提供生长优势。我们还发现，迄今为止功能未知的质周蛋白PA0808可提供自我保护，免受AmpDh3的裂解。AmpDh3-PA0808对同源基因广泛分布于革兰氏阴性菌中。综上所述，这些发现强调了它们作为进化优势的功能的重要性，以及H2-T6SS作为表现这种效应的手段的重要性。
The human pathogen Pseudomonas aeruginosa harbors three paralogous zinc proteases annotated as AmpD, AmpDh2, and AmpDh3, which turn over the cell wall and cell-wall-derived muropeptides. AmpD is cytoplasmic and plays a role in recycling of cell-wall muropeptides, with a link to antibiotic resistance. AmpDh2 is a periplasmic soluble enzyme with the former anchored to the inner leaflet of the outer membrane. We document herein that the type VI secretion system locus II (H2-T6SS) of P. aeruginosa delivers AmpDh3 (but not AmpD or AmpDh2) to the periplasm of a prey bacterium upon contact. AmpDh3 hydrolyzes the cell-wall peptidoglycan of the prey bacterium, which leads to its killing, thereby providing a growth advantage for P. aeruginosa in bacterial competition. We also document that the periplasmic protein PA0808, heretofore of unknown function, affords self-protection from lysis by AmpDh3. Cognates of the AmpDh3-PA0808 pair are widely distributed across Gram-negative bacteria. Taken together, these findings underscore the importance of their function as an evolutionary advantage and that of the H2-T6SS as the means for manifestation of the effect.
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影响因子：
30.3
作者：
Ma LS;Hachani A;Lin JS;Filloux A;Lai EM
通讯作者：
Lai EM
影响因子：
14.9
作者：
Liang H;Deng X;Li X;Ye Y;Wu M
通讯作者：
Wu M
影响因子：
3.4
作者：
Imperi, Francesco;Ciccosanti, Fabiola;Fimia, Gian Maria
通讯作者：
Fimia, Gian Maria
影响因子：
28.3
作者：
Burkinshaw, Brianne J.;Liang, Xiaoye;Dong, Tao G.
通讯作者：
Dong, Tao G.
DOI：
10.1098/rstb.2015.0021
发表时间：
2015-10-05
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Basler M
通讯作者：
Basler M