Agrobacterium tumefaciens deploys a superfamily of type VI secretion DNase effectors as weapons for interbacterial competition in planta.
Agrobacterium tumefaciens deploys a superfamily of type VI secretion DNase effectors as weapons for interbacterial competition in planta.
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DOI：
10.1016/j.chom.2014.06.002
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发表时间：
2014-07-09
影响因子：
30.3
通讯作者：
Lai EM
中科院分区：
文献类型：
--
作者：
Ma LS;Hachani A;Lin JS;Filloux A;Lai EM
VI型分泌系统(T6SS)是一种广泛的分子武器，被许多变形杆菌利用来靶向真核和原核细胞中的效应物/毒素。我们报道了根癌农杆菌，一种在植物中触发肿瘤发生的土壤细菌，产生一种不同于先前已知的多态毒素和核酸酶的VI型DNA酶效应因子(TDE)家族。TDE显示出依赖于保守的HxxD基序的抗细菌DNA酶活性，并可被同源免疫蛋白TDI所抵消。在体外，根癌农杆菌T6SS可以杀死大肠杆菌，但在注射TDE毒素时会引发铜绿假单胞菌的致命反击。然而，在一项植物内联合感染试验中，根癌农杆菌使用TDE效应器攻击同胞细胞和铜绿假单胞菌，最终获得竞争优势。这种在体内获得的T6SS依赖的适应性和TDE-TDI对在细菌中的保守突显了一种有利于利基定植的广泛的抗菌武器。在根癌农杆菌TDE中发现的VI型分泌DNase效应子(TDE)家族具有抗菌DNA酶活性，并被免疫蛋白抵消，TDI超家族在宿主定植过程中对根癌农杆菌具有竞争优势。TDI超家族在细菌中广泛保守。Ma等人。报告发现了一类依赖T6SS的DNA酶，它们支持植物病原菌根癌农杆菌在植物定植期间对竞争对手(如铜绿假单胞菌)的生存和适应性。
The type VI secretion system (T6SS) is a widespread molecular weapon deployed by many Proteobacteria to target effectors/toxins into both eukaryotic and prokaryotic cells. We report that Agrobacterium tumefaciens, a soil bacterium that triggers tumorigenesis in plants, produces a family of type VI DNase effectors (Tde) that are distinct from previously known polymorphic toxins and nucleases. Tde exhibits an antibacterial DNase activity that relies on a conserved HxxD motif and can be counteracted by a cognate immunity protein, Tdi. In vitro, A. tumefaciens T6SS could kill Escherichia coli but triggered a lethal counterattack by Pseudomonas aeruginosa upon injection of the Tde toxins. However, in an in planta coinfection assay, A. tumefaciens used Tde effectors to attack both siblings cells and P. aeruginosa to ultimately gain a competitive advantage. Such acquired T6SS-dependent fitness in vivo and conservation of Tde-Tdi couples in bacteria highlights a widespread antibacterial weapon beneficial for niche colonization. A type VI secretion DNase effector (Tde) family identified in Agrobacterium tumefaciens Tde exhibits antibacterial DNase activity and is counteracted by immunity protein Tdi Tde confers a competitive advantage to A. tumefaciens during host colonization Tde and Tdi superfamilies are broadly conserved in bacteria Type VI secretion systems (T6SSs) are weapons involved in interbacterial competition. Ma et al. report the discovery of a class of T6SS-dependent DNases that support the survival and fitness of the plant pathogen Agrobacterium tumefaciens against competitors such as Pseudomonas aeruginosa during plant colonization.
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影响因子：
6.7
作者：
Alteri CJ;Himpsl SD;Pickens SR;Lindner JR;Zora JS;Miller JE;Arno PD;Straight SW;Mobley HL
通讯作者：
Mobley HL
影响因子：
3.6
作者：
Kapitein, Nicole;Boenemann, Gabriele;Mogk, Axel
通讯作者：
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DOI：
10.1073/pnas.1300627110
发表时间：
2013-04-23
影响因子：
11.1
作者：
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通讯作者：
Hayes, Christopher S.
影响因子：
64.5
作者：
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通讯作者：
Mekalanos JJ
影响因子：
2.8
作者：
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通讯作者：
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