Plastid transformation in Arabidopsis thaliana
Plastid transformation in Arabidopsis thaliana
复制标题
拟南芥质体转化
DOI：
10.1007/s002990050525
复制
发表时间：
2019
影响因子：
6.2
通讯作者：
P. Maliga
中科院分区：
文献类型：
--
作者：
S. Sikdar;G. Serino;S. Chaudhuri;P. Maliga
在将DNA转化到叶细胞的生物传递之后，报道了拟南芥中的叶绿体转化。转化质粒pGS31a携带一个壮观霉素抗性(AADA)基因，其两侧带有质体DNA序列，目的是将其插入到entnV和therps12/7操纵子之间。通过侧翼ptDNA序列的两个同源重组事件整合faadA，并在壮观霉素培养基上选择性扩增转体，获得了抗性细胞系和再生植株，其中的叶绿体基因组拷贝发生了均匀的变化。转化效率较低，201个叶片样品中只有2个。从这两个品系再生的98株植株中没有一株是可育的。
Plastid transformation is reported inArabidopsis thalianafollowing biolistic delivery of transforming DNA into leaf cells. Transforming plasmid pGS31A carries a spectinomycin resistance (aadA) gene flanked by plastid DNA sequences to target its insertion betweentrnVand therps12/7operon. Integration ofaadAby two homologous recombination events via the flanking ptDNA sequences and selective amplification of the transplastomes on spectinomycin medium yielded resistant cell lines and regenerated plants in which the plastid genome copies have been uniformly altered. The efficiency of plastid transformation was low: 2 in 201 bombarded leaf samples. None of the 98 plants regenerated from the two lines were fertile.
影响因子：
11.4
作者：
SHINOZAKI, K;OHME, M;SUGIURA, M
通讯作者：
SUGIURA, M
影响因子：
11.6
作者：
MELARAGNO, JE;MEHROTRA, B;COLEMAN, AW
通讯作者：
COLEMAN, AW