Epigallocatechin-3-gallate (EGCG) protects skin cells from ionizing radiation via heme oxygenase-1 (HO-1) overexpression.
Epigallocatechin-3-gallate (EGCG) protects skin cells from ionizing radiation via heme oxygenase-1 (HO-1) overexpression.
复制标题
表没食子儿茶素-3-没食子酸酯 (EGCG) 通过血红素加氧酶-1 (HO-1) 过度表达保护皮肤细胞免受电离辐射。
DOI：
10.1093/jrr/rru047
复制
发表时间：
2014-11
影响因子：
2
通讯作者：
Cao J
中科院分区：
文献类型：
--
作者：
Zhu W;Xu J;Ge Y;Cao H;Ge X;Luo J;Xue J;Yang H;Zhang S;Cao J
表没食子儿茶素-3-没食子酸酯（EGCG）是绿色茶的主要多酚成分，是一种有效的抗氧化剂和自由基清除剂，可用于治疗许多疾病。放射疗法被广泛用于治疗各种类型的癌症;然而，辐射引起的皮肤损伤仍然是一个严重的问题。EGCG尚未被报道为保护皮肤细胞免受电离辐射。在本研究中，我们调查是否表没食子儿茶素没食子酸酯赋予电离辐射的细胞保护。我们发现，与对照组相比，预处理与EGCG显着提高了人皮肤细胞的活力与X射线照射，并减少由X射线照射诱导的细胞凋亡。Mito-Tracker实验表明，EGCG通过上调SOD 2的表达，抑制电离辐射对线粒体的损伤。照射前用EGCG预处理可显著降低HaCaT细胞内的活性氧（ROS）。辐射诱导的γ H2 AX灶，这是DNA双链断裂的代表，减少与EGCG预处理。此外，EGCG通过转录激活以剂量依赖性方式诱导细胞保护分子血红素加氧酶-1（HO-1）的表达。HO-1敲低或用HO-1抑制剂锡原卟啉（SnPPIX）处理逆转了EGCG的保护作用，表明HO-1的重要作用。这些结果表明，表没食子儿茶素没食子酸酯提供了一种新的策略，保护皮肤免受电离辐射。
Epigallocatechin-3-gallate (EGCG), the major polyphenolic constituent of green tea, is a potent antioxidant and free radical scavenger that may have therapeutic applications for the treatment of many disorders. Radiation therapy is widely used for the treatment of various types of cancers; however, radiation-induced skin injury remains a serious concern. EGCG has not yet been reported as protecting skin cells against ionizing radiation. In the present study, we investigated whether EGCG confers cytoprotection against ionizing radiation. We found that, compared with the control, pretreatment with EGCG significantly enhanced the viability of human skin cells that were irradiated with X-rays, and decreased apoptosis induced by X-ray irradiation. Mito-Tracker assay showed that EGCG suppressed the damage to mitochondria induced by ionizing radiation via upregulation of SOD2. Reactive oxygen species (ROS) in HaCaT cells were significantly reduced when pretreated with EGCG before irradiation. Radiation-induced γH2AX foci, which are representative of DNA double-strand breaks, were decreased by pretreatment with EGCG. Furthermore, EGCG induced the expression of the cytoprotective molecule heme oxygenase-1 (HO-1) in a dose-dependent manner via transcriptional activation. HO-1 knockdown or treatment with the HO-1 inhibitor tin protoporphyrin (SnPPIX) reversed the protective role of EGCG, indicating an important role for HO-1. These results suggest that EGCG offers a new strategy for protecting skin against ionizing radiation.
登录
查看更多内容
影响因子：
3.7
作者：
Barjaktarovic Z;Schmaltz D;Shyla A;Azimzadeh O;Schulz S;Haagen J;Dörr W;Sarioglu H;Schäfer A;Atkinson MJ;Zischka H;Tapio S
通讯作者：
Tapio S
HIV-TAT 介导的铜/锌超氧化物歧化酶-1 (SOD1) 蛋白转导可保护皮肤细胞免受电离辐射。
DOI：
10.1186/1748-717x-8-253
发表时间：
2013-10-31
期刊：
Radiation oncology (London, England)
影响因子：
--
作者：
Gu Q;Feng T;Cao H;Tang Y;Ge X;Luo J;Xue J;Wu J;Yang H;Zhang S;Cao J
通讯作者：
Cao J
影响因子：
3.6
作者：
Baskar R;Lee KA;Yeo R;Yeoh KW
通讯作者：
Yeoh KW
DOI：
10.1124/jpet.103.059220
发表时间：
2004-02-01
影响因子：
3.5
作者：
Ahmed, S;Wang, NZ;Haqqi, TM
通讯作者：
Haqqi, TM
影响因子：
4.2
作者：
Frei, B;Higdon, JV
通讯作者：
Higdon, JV